Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Oxidoreductases
Patent
1996-01-04
1999-02-23
Marx, Irene
Drug, bio-affecting and body treating compositions
Enzyme or coenzyme containing
Oxidoreductases
435189, 435 4, 435 26, A61K 5844
Patent
active
058740783
ABSTRACT:
A novel glutamate dehydrogenase derived from the genus Pseudomonas which is specific to L-glutamic acid, requires NAD.sup.+ and NADH as coenzymes, does not act on NADP.sup.+ and NADPH, is with an optimum pH 10.5-11.5 (oxidative deamination), a pH stability of 5-10 (at 25.degree. C.; 20 hours), an optimum temperature of about 60.degree. C. (oxidative deamination) and a molecular weight of about 280,000 (gel filtration) or about 41,000 (SDS-PAGE) and is not activated by ADP. A reagent composition for measuring an amount of ammonia characterized in containing a glutamate dehydrogenase which is not activated by ADP and is NADH-dependent, .alpha.-ketoglutaric acid or a salt thereof and NADH; and a reagent composition wherein urease or creatinine deiminase is further added to the above-mentioned reagent composition.
REFERENCES:
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Hattori Shizuo
Hongo Noriyuki
Kawamura Yoshihisa
Teshima Shin'ichi
Yamamoto Kazumi
Marx Irene
Toyo Boseki Kabushiki Kaisha
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