Reagent combination for solid phase immunofluorescent assay

Details Reagent combination for solid phase immunofluorescent assay Reagent combination for solid phase immunofluorescent assay

1979-10-04

1981-03-03

Fagelson, Anna P.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 424 7, 424 11, 424 12, 424 13, 424 78, G01N 3352, G01N 3354

Patent

active

042540968

ABSTRACT:
An assay method suitable for antigens (or haptens) including proteins and polypeptides utilizing antibodies for the unknown protein or polypeptide in which the antibodies are covalently bound to water insoluble hydrophilic polymeric particles. Appropriate fluorescently labeled immune reactants are introduced during the method and together with the unknown antigen are immunologically bound, directly or indirectly, to the particles for separation from the reaction liquid. The particles have a size of about 0.1-10 microns and can be resuspended for direct measurement of the fluorescent labels in a fluorometer from which the unknown can be determined.

REFERENCES:
patent: 3555143 (1971-01-01), Axen
patent: 3641235 (1972-02-01), Weiss
patent: 3720760 (1973-03-01), Bennick
patent: 3853987 (1974-12-01), Dreyer
patent: 3901654 (1975-08-01), Gross
patent: 3925018 (1975-12-01), Saunders
patent: 3998943 (1976-12-01), Ullman
Molday, Nature, vol. 249, May 3, 1974, pp. 81-83.
Capel, J. Immunol. Methods, vol. 5, 1974, pp. 165-178.

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