Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-12-29
2009-12-29
Gabel, Gailene R (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S002000, C435S006120, C435S007250, C435S285200, C435S173500, C435S173700, C436S522000, C436S546000, C436S008000, C436S010000, C436S017000, C436S018000, C436S172000, C436S175000, C436S176000, C422S068100, C422S073000, C422S082020, C422S082050, C422S082070, C422S082090, C422S098000
Reexamination Certificate
active
07638290
ABSTRACT:
The invention relates to a reagent and a process for the identification and counting of biological cells in a sample. This reagent comprises a cell lysing agent selected from at least one detergent in a concentration capable of specifically lysing a given type of cells in the sample, and a stain capable of marking the intracellular nucleic acids of the remaining unlysed cells. Application in particular for the identification and counting of cells using an automated analysis system based on flow cytometry.
REFERENCES:
patent: 4751179 (1988-06-01), Ledis et al.
patent: 5232857 (1993-08-01), Lefevre et al.
patent: 5496734 (1996-03-01), Sakata
patent: 5648225 (1997-07-01), Kim et al.
patent: 5939326 (1999-08-01), Chupp et al.
patent: 5994138 (1999-11-01), Veriac
patent: 6271035 (2001-08-01), Deka et al.
patent: 6562563 (2003-05-01), Murphy et al.
patent: 0 343 380 (1989-04-01), None
patent: 2 759 166 (1998-08-01), None
Haas et al., Cation-Anion Cotransport, Methods in Enzymology, 173:280-91 (1989) Abstract.
Lawrence R. Adams, et al., “Fluorometric Characterization of Six Classes of Human Leukocytes” Acta Cytol, vol. 18, No. 5, pp. 389-391, 1974.
Howard M. Shapiro et al., “Combined Blood Cell Counting and Classification with Fluorochrome Stains and Flow Instrumentation”, J. Histochem Cytochem, vol. 24, No. 1, pp. 396-411, 1976.
Leon W.M.M. Terstappen et al., “Multidimensional Flow Cytometric Blood Cell Differentiation Without Erythrocyte 30 Lysis”, Blood Cells, vol. 17, pp. 585-602, 1991.
Leon W.M.M. Terstappen et al., “Bone Marrow Cell Differential Counts Obtained by Multidimensional Flow Cytometry”, Blood Cells, vol. 18 (2), pp. 311-330, 1992.
F. Traganos, et al., “Simultaneous Staining of Ribonucleic and Deoxyribonucleic Acids in Unfixed Cells Using Acridine Orange in a Flow Cytofluorometric System”, The Journal of Histochemistry and Cytochemistry, vol. 25, No. 1, pp. 46-56, 1977.
Alan Pollack et al., “Flow Cytometric Analysis of RNA Content in Different Cell Populations Using Pyronin Y and Methyl Green”, Cytometry, vol. 3, No. 1, pp. 28-35, 1982.
Champseix Henri
Lefevre Didier
Veriac Sylvie
ABX
Gabel Gailene R
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