Reagent and method for the determination of peroxidase

Details Reagent and method for the determination of peroxidase Reagent and method for the determination of peroxidase

1980-02-11

1981-11-24

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

23230B, 252408, 260326C, 435 28, C12Q 166

Patent

active

043025374

ABSTRACT:
The present invention provides a method for the determination of peroxidase in the presence of a compound, the enzyme-catalysed oxidation of which with a peroxy compound results in the emission of light, which is measured, wherein 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide of the formula ##STR1## is used as light-emitting compound. The present invention also provides a reagent for the determination of peroxidase, comprising 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide, a system providing hydrogen peroxide and a buffer substance of pH 6 to 9.

REFERENCES:
patent: 3654179 (1972-04-01), Bauer
patent: 3986833 (1976-10-01), Mast et al.

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