Reagent and method for the determination of cholesterol

Details Reagent and method for the determination of cholesterol Reagent and method for the determination of cholesterol

1973-05-15

1980-07-15

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 19, 435291, C12Q 150

Patent

active

042129389

ABSTRACT:
Cholesterol is determined by incubating a sample suspected of containing cholesterol in an aqueous medium with cholesterol oxidase and determining either the oxygen consumption, the hydrogen peroxide formed, or cholestenone, as a measure of the initial cholesterol content; reagent compositions comprising cholesterol oxidase and a system for the determination of H.sub.2 O.sub.2 or for the determination of cholestenone are provided.

REFERENCES:
patent: 3099605 (1963-07-01), Free
patent: 3282803 (1966-11-01), Poepel et al.
patent: 3607093 (1971-09-01), Stone
patent: 3776816 (1973-12-01), Terada et al.
Schatz et al., J. Bact., vol. 58, pp. 117-125 (1949).
Colowick et al., Methods in Enzymology, vol. I, pp. 678-681 (1955).
Heftmann et al., Biochemistry of Steroids, pp. 14-16 (1960).

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