Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-06-06
2000-01-25
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C07H 2102, C07H 2104, C12N 1500
Patent
active
060177071
ABSTRACT:
Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods include a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.
REFERENCES:
patent: 4687732 (1987-08-01), Ward et al.
patent: 4731325 (1988-03-01), Palva et al.
patent: 4868105 (1989-09-01), Urdea et al.
patent: 4882269 (1989-11-01), Schneider et al.
patent: 4894325 (1990-01-01), Englehardt et al.
patent: 4925785 (1990-05-01), Wang et al.
patent: 5104791 (1992-04-01), Abbott et al.
patent: 5124246 (1992-06-01), Urdea et al.
patent: 5273882 (1993-12-01), Snitman et al.
patent: 5374524 (1994-12-01), Miller
patent: 5387510 (1995-02-01), Wu
patent: 5424188 (1995-06-01), Schneider et al.
patent: 5695936 (1997-12-01), Mandrand et al.
Nicholls et al., Journal of Clinical Laboratory Analysis 3 : 122-135 (1989).
The Stragene Catalog, p. 39 (1988).
Richard D. Abramson et al., "Nucleic Acid Amplification Technologies", Current Opinion in Biotechnology, pp. 41-47, May 14, 1993.
Konrad Misiura et al., "Biotinyl and Phosphotyrosinyl Phosphoramidite Derivatives Useful in the Incorporation of Multiple Reporter Groups on Synthetic Oligonucleotides", Nucleic Acids Research, vol. 18, No. 15, pp. 4345-4354, 1990.
Uwe Pieles et al., "A Protected Biotin Containing Deoxycytidine Building Block for Solid Phase Synthesis of Biotinylated Oligonucleotides", vol 18, No. 15, pp. 4355-4360, 1990.
Ricki Lewis, "PCR's Competitors are Alive and Well and Moving Rapidly Towards Commercialization", Genetic Engineering News, vol. 12, No. 9, pp. 1, 8-9, Jun. 1, 1992.
Sommer et al., Nucleic Acids Research 17(16) : p. 6749 (1989).
Charles Marie-Helene
Cros Philippe
Delair Thierry
Erout Marie-Noelle
Mandrand Bernard
Bio Merieux
Jones W. Gary
Whisenant Ethan
LandOfFree
Reagent and method for the detection of a nucleotide sequence wi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Reagent and method for the detection of a nucleotide sequence wi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagent and method for the detection of a nucleotide sequence wi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2314910