Reagent and method for measuring amylase

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

Reexamination Certificate

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Reexamination Certificate

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10379094

ABSTRACT:
An object of the present invention is to provide a means for the measurement of amylase activity that exists in a biological sample such as saliva in a more convenient manner and particularly to provide a means (method and reagent) where a sample containing amylase in high concentration is directly measured without dilution. In the present invention, there has been found a method, which is an enzymatic method using a modified oligosaccharide substrate, comprising adding saccharide such as oligosaccharide that is competitive to the oligosaccharide substrate whereupon amylase activity in an amylase sample having a high activity value can be directly measured without dilution, and a result, the present invention has been achieved.

REFERENCES:
patent: 4622295 (1986-11-01), Ikenaka et al.
patent: 4810636 (1989-03-01), Corey
patent: 5077011 (1991-12-01), Amano et al.
patent: 5223219 (1993-06-01), Subramanian et al.
patent: 5334502 (1994-08-01), Sangha
patent: 5384245 (1995-01-01), Kwan
patent: 2003/0175843 (2003-09-01), Yamaguchi et al.
patent: 0 324 912 (1989-07-01), None
patent: 0 459 536 (1991-12-01), None
patent: 0 557 021 (1993-08-01), None
Majima et al, “Determination of alpha-amylase using a new blocked substrate”, 1995 Clinica Chimica Acta, vol. 234, pp. 177-179.
Young et al, “Use of a Competitive Inhibitor in a Kinetic Enzymatic Method for Measuring Ethanol in Serum”, 1987 Clinical Chemistry, vol. 33/12, pp. 2296-2298.
Chatterton et al., “Salivary α-amylase as a Measure of Endogenous Adrenergic Activity,” Clinical Physiology, No. 16, 1996, pp. 433-448.
Speirs et al., “The Influence of Sympathetic Activity and Isoprenaline on the Secretion of Amylase from the Human Parotid Gland,” Archives of Oral Biology, vol. 19, 1974, pp. 747-752.
Chrousos et al., “The Concepts of Stress and Stress System Disorders,” Journal of the American Medical Association, vol. 267, No. 9, Mar. 4, 1992, pp. 1244-1252.
“Flow-injection-type Biosensor System for Salivary Amylase Activity” Biosensors 2002, May 16, 2002.
“Change of Amylase activity on physical stress test” The Japanese Journal of Stress sciences Oct. 29, 2002 (vol. 16(2)).
“Is Saliva an Index for stress Level?” Iyou densi seitaikougaku, vol. 39, No. 3 (2002).
“Stress Monitor Via salivary Amylase Activity” CMC syuppankan Monthly Bio Industry-2002 vol. 10 (10) p. 20-25.
“Analysis of stress reaction by salivary α-amylase” Dai 45 kai nihon daekisen gakkai syourokusyu, vol. 42, 2001.
Toyama daigaku kouhousi Tomuzu magazinn, No. 4 winter 2002.
Toyama News Paper Mar. 2, 2001.
Nihon keizai News Paper Mar. 23, 2001.
Yomiuri News Paper May 2, 2001.
Toyama News Paper Apr. 5, 2002.
Kitanihon News Paper Jul. 10, 2002.
Japanese Journal of Clinical Dentistry of Children Apr. 2002 (vol. 7, No. 4) p. 35-39.
Suganuma T et al., “Study of the action of human salivary alpha-amylase on 2-chloro-4-nitrophenyl alpha-maltotrioside in the present of potassium thiocyanate”,Carbohydrate Reasearch, Elseview Scientific Publishing Company. Amsterdam, NL vol. 303, No. 2, Sep. 5, 1997.
Winn-Deen E S et al., “Developmental of a direct assay for X-amylase”,Clinical Chemistry, Amercican Association for Clinical Chemistry. Winston, US vol. 34, No. 10, Oct. 1, 1988.

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