Reagent and method for isolation and detection of selected nucle

Details Reagent and method for isolation and detection of selected nucle Reagent and method for isolation and detection of selected nucle

1997-11-13

2000-05-09

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C07H 2102, C07H 2104, C12N 1500

Patent

active

060602465

ABSTRACT:
The invention relates to compositions and methods for rapidly detecting or isolating a target nucleic acid sequence in a polynucleotide-containing sample. The sample is exposed to a rapid pairing reagent, which contains a rapid capture component, effective to rapidly and non-selectively bind polynucleotides, and a target specific probe, effective to selectively bind the target nucleic acid sequence. Selectively disrupting the binding between the capture component and polynucleotides leaves only target sequence bound to the rapid pairing reagent.

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Kuribayashi-Ohta, Keiko, et al., "Application of oligo(dT).sub.30 -latex for rapid purification of poly(A).sup.+ mRNA and for hybrid subtraction with the in situ reverse transcribed cDNA," Biochemica et Biophysica Acta 1156:204-212 (1993).
Lund, Vera, et al., "Assessment of methods for covalent binding of nucleic acids to magnetic beads, Dynabeads.TM. and the characteristics of the bound nucleic acids in hybridization reactions," Nuc. Acids, Res. 16(22):10861-10881 (1988).
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