Chemistry: analytical and immunological testing – Biological cellular material tested
Patent
1997-11-04
1998-12-01
Soderquist, Arlen
Chemistry: analytical and immunological testing
Biological cellular material tested
436 8, 436 10, 436 17, 436 18, G01N 3348, G01N 3100
Patent
active
058436081
ABSTRACT:
A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enable the differentiation of at least two subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system find use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an ethoxylated long chain compound based lysis reagent with an acid and a solubilizer, and a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples, as well as other fluid samples such as bone marrow.
REFERENCES:
patent: 3741875 (1973-06-01), Ansley et al.
patent: 4028268 (1977-06-01), Sullivan, 3rd et al.
patent: 4121898 (1978-10-01), Kirschnek et al.
patent: 4286963 (1981-09-01), Ledis et al.
patent: 4396674 (1983-08-01), Somezawa et al.
patent: 4485175 (1984-11-01), Ledis et al.
patent: 4525296 (1985-06-01), Quinlan
patent: 5116539 (1992-05-01), Hamaguchi et al.
patent: 5125737 (1992-06-01), Rodriquez et al.
patent: 5155044 (1992-10-01), Ledis et al.
patent: 5196346 (1993-03-01), Lefevre et al.
patent: 5198485 (1993-03-01), King et al.
patent: 5242832 (1993-09-01), Sakata
patent: 5250437 (1993-10-01), Toda et al.
patent: 5389549 (1995-02-01), Hamaguchi et al.
patent: 5618733 (1997-04-01), Sakata et al.
patent: 5686308 (1997-11-01), Li et al.
Fauci, A.S. et al. (1991) Immunopathogenic Mechanisms in Human Immunodeficiency Virus (HIV) Infection. Ann. Intern. Med. 114(8): 678-692.
Malech, H.L. et al. (1978) Neutrophils in Human Diseases. N. Engl. J. Med. 317(11): 687-694.
Porter, M.R. (1991) Handbook of Surfactants. Chapman and Hall, New York.
Carter James H.
Fischer Timothy J.
Li Yi
Veulens Sergio C.
Young Carole
Alter Mitchell E.
Coulter International Corp.
Soderquist Arlen
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