Reagent and method for differential determination of leukocytes

Details Reagent and method for differential determination of leukocytes Reagent and method for differential determination of leukocytes

1995-12-18

1998-10-06

Wallenhorst, Maureen M.

Chemistry: analytical and immunological testing

Biological cellular material tested

436 8, 436 10, 436 17, 436164, 436166, 436171, 436175, 2524081, 435 2, G01N 3348

Patent

active

058175180

ABSTRACT:
This invention relates to a lytic reagent and a method of using the lytic reagent for automatically determining leukocyte subpopulations in blood. More specifically, the new lytic reagent lyses red blood cells and affects the eosinophils which enables the differentiation of at least one subpopulation of leukocytes. The lytic reagent is an acidic, hypertonic aqueous solution containing alkali metal salt of alkyl sulfite, an eosinolytic agent, a nonionic surfactant and a physiological salt. When used in combination with a second lytic reagent system, one is able to obtain at least a five part differential of leukocytes using DC and RF measurements.

REFERENCES:
patent: 2656508 (1953-10-01), Coulter
patent: 4298836 (1981-11-01), Groves et al.
patent: 4330341 (1982-05-01), May et al.
patent: 4485175 (1984-11-01), Ledis et al.
patent: 4751179 (1988-06-01), Ledis et al.
patent: 5116539 (1992-05-01), Hamaguchi et al.
patent: 5125737 (1992-06-01), Rodriguez et al.
patent: 5155044 (1992-10-01), Ledis et al.
patent: 5196346 (1993-03-01), Lefevre et al.
patent: 5232857 (1993-08-01), Lefevre et al.
patent: 5389549 (1995-02-01), Hamaguchi et al.
patent: 5496734 (1996-03-01), Sakata
patent: 5510267 (1996-04-01), Marshall
patent: 5538893 (1996-07-01), Sakata et al.

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