Reagent and method for determining activity of herpes protease

Details Reagent and method for determining activity of herpes protease Reagent and method for determining activity of herpes protease

1994-04-29

1996-04-09

Warden, Jill

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 24, 435 5, 530326, 530327, 530328, 530329, 530330, C07K 500, C07K 700, C07K 1700, C12Q 137

Patent

active

055061150

ABSTRACT:
A class of compounds is described for use as fluorogenic substrates for herpesvirus proteases and for use in an assay to identify inhibitors of herpesvirus proteases. Compounds of particular interest are defined by Formula II ##STR1## wherein W is selected from glycine, 4-aminobutyric acid, 5-aminopentanoic acid, 6-aminocaproic acid and 7-aminoheptanoic acid; wherein Y is selected from EDANS, Abz, DANSYL, nicotinic acid, 4-guanidino-benzoic acid, N-methyl-Abz, 4-chloro-Abz, 5-chloro-Abz, 6-chloro-Abz, 3,5-dibromo-Abz, 6-amino-nicotinic acid, 2-aminonicotinic acid, 2-chloronicotinic acid, niflumic acid and fluorogenic derivatives thereof; and wherein Z is selected from tryptophan, tyrosine, phenylalanine, p-nitrophenylalanine, m-nitrophenylalanine, DABSYL, DABCYL and halogenated derivatives thereof.

REFERENCES:
patent: 5011910 (1991-04-01), Marshall et al.
Ljungman et al., J. Infect. Dis., 162, 244 (1990).
Gately et al, J. infect. Dis., 161, 711 (1990).
Lui and Roizman, J. Virol., 65, 5149 (1991).
A. R. Welch et al., Proc. Natl. Acad. Sci USA, 88, 10792 (1991).
G. Guilbault, Enzymatic Methods of Analysis, 43-47 (1970).
S. Netzel-Arnett et al, Anal. Biochem., 195, 86 (1991).
C. Garcia-Echeverria, FEBS Lett., 297, 100 (1992).
C. Garcia-Echeverria, Biochem. and Biophys. Res. Comm., 187, 615 (1992).
E. Matayoshi et al, Science, 247, 954 (1990).
G. Wang et al, Tet. Lett., 31, 6493 (1990).
L. Maggiora et al, J. Med. Chem, 35, 3727 (1992).

Affiliated with

Also associated with

Rating

Reagent and method for determining activity of herpes protease does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Reagent and method for determining activity of herpes protease, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagent and method for determining activity of herpes protease will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-138136