Reagent and method for determination of globulin

Details Reagent and method for determination of globulin Reagent and method for determination of globulin

1975-07-11

1976-10-12

Wolk, Morris O.

Chemistry: physical processes

Physical processes

Crystallization

252408, G01N 2102, G01N 3316

Patent

active

039855066

ABSTRACT:
A stable reagent for use in a rapid and sensitive globulin estimation procedure consists of 60-70 volumes of glacial acetic acid, 1-20 volumes of monomethyl ether of ethylene glycol, 5-15 volumes of phosphoric acid, 5-15 volumes of sulfuric acid, 0.2-0.6 volumes of twenty percent glyoxylic acid in aqueous solution, and 0.4-1.2 volumes of ten pecent ferric chloride hexahydrate in aqueous solution. The reagent is far more stable, sensitive, and tolerant of water in the reaction mixture than previously known reagents used in the glyoxylic reaction.

REFERENCES:
patent: 3607081 (1971-09-01), Goldenberg
patent: 3627468 (1971-12-01), Goldenberg et al.
Dalby et al., Anal. Biochem., v. 63, pp. 283-285 (1975).

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