Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-04-14
2008-08-12
Nguyen, Bao-Thuy L (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S070200, C435S070210, C435S007920, C435S007940, C435S007950, C436S547000, C436S548000, C530S388100, C530S388600, C530S389100, C530S822000, C530S864000, C424S130100, C424S141100, C424S151100, C424S265100, C424S543000
Reexamination Certificate
active
07410771
ABSTRACT:
A reagent and method for the specific and highly sensitive detection ofC. parvumin which the reagent is an antibody for a solubleC. parvumsporozoite antigen and the method is an immunoassay in which the antibody is used to detect or quantifyC. parvumsporozoite antigen in a sample. The sample is treated to cause excystation ofC. parvumoocytes, thereby releasing aC. parvumsporozoite antigen, and combined with antibodies specific for the sporozoite antigen under conditions to form an antibody-antigen complex. Detection of the complex indicates the presence ofC. parvumin the sample. The assay allows recognition and detection ofC. parvumin turbid samples, and due to a lack of crossreactivity with otherCryptosporidiumspecies, is specific forC. parvumcontamination or infection. The assay is highly sensitive, allowing for the detection of less than 100 oocysts per milliliter of sample.
REFERENCES:
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Call Jeffrey L.
Hancock Kathy
Lee Yeuk-mui
Tsang Victor C. W.
Klarquist & Sparkman, LLP
Nguyen Bao-Thuy L
The United States of America as represented by the Department of
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