Reagent and method for classification and counting of leukocytes

Details Reagent and method for classification and counting of leukocytes Reagent and method for classification and counting of leukocytes

1998-05-15

1999-12-21

Wallenhorst, Maureen M.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

436 8, 436 17, 436 63, 436164, 436172, 436800, 2524081, G01N 3100

Patent

active

060048166

ABSTRACT:
A reagent for classification and counting of leukocytes, the reagent containing at least one dye which has the following structural formula ##STR1## where R.sub.1 is a hydrogen atom or an alkyl group, R.sub.2 and R.sub.3 each represent a hydrogen atom, a lower alkyl group or a lower alkoxy group, R.sub.4 represents a hydrogen atom, an acyl group or an alkyl group, Z represents a sulfur atom, an oxygen atom, or a carbon atom having a lower alkyl group, n denotes 0, 1 or 2, and X.sup.- represents an anion,
and specifically binds to RNA to increase in fluorescence intensity, and a method for classification and counting of leukocytes which uses the reagent, whereby abnormal cells such as immature leukocytes and abnormal leukocytes can be classified and counted easily and highly accurately, and at the same time, classification and counting of normal leukocytes as well as the counting of leukocytes can be performed.

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