Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1997-10-16
1999-09-14
Duffy, Patricia A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
530810, 530811, 530812, 530813, 530814, 435 71, 435975, C01K 1600, G01N 3353
Patent
active
059524731
ABSTRACT:
The invention provides an immobilised oligopeptide for detecting protein prenylation consisting of an oligopeptide containing the amino acid sequence Xad-Xac-Xab-Xaa-OH at its carboxyl-terminus, at least one of Xaa, Xab, Xac and Xad representing cysteine (Cys), said sequence being capable of acting as a substrate for a prenyl transferase catalysing protein prenylation, and being bonded to a solid carrier, preferably at its amino-terminus. The invention further provides a kit for detecting protein prenylation comprising a first immobilised oligopeptide as mentioned above and a second immobilised oligopeptide which differs from said first immobilised oligopeptide in that the cysteine residue is substituted by another amino acid. Also provided are antibodies against the prenylated oligopeptides.
REFERENCES:
patent: 5141851 (1992-08-01), Brown et al.
patent: 5185248 (1993-02-01), Barbacid et al.
patent: 5366871 (1994-11-01), Rechsteiner et al.
patent: 5420245 (1995-05-01), Brown et al.
Campbell, in Laboratory Techniques in Biochemistry and Molecular Biology, pub 1991 by Elsevier, NY. pp. 1-49.
Hermanson et al "Immobilized Affinity Ligand Techniques", 1992 by Academic Press Inc, San Diego CA, pp. 137-158.
"A protein geranylgeranyltransferase from bovine brain: Implications for protein prenylation specificity", Proceedings of the National Academy of Sciences of USA, vol. 88, Jun. 1991, Washington by K. Yokoyama et al., pp. 5302-5306.
"In vitro identification of a soluble protein: geranylgeranyl transferase from rat tissues", The Journal of Biological Chemistry, vol. 266, No. 21, Jul 25, 1991, Baltimore, by A. Joly et al., pp. 13495-13498.
"Sequence dependence of protein isoprenylation", The Journal of Biological Chemistry, vol. 266, No. 22, Aug. 5, 1991, Baltimore by S.L. Moores et al., pp. 14603-14610.
"Nonfarnesylated tetrapeptide inhibitors of protein farnesyltransferase", The Journal of Biological Chemistry, vol. 266, No. 24, Aug. 25, 1991, Baltimore, by J.L. Goldstein et al., pp. 15575-15578.
"Biochemistry of protein prenylation", Journal of Lipid Research, vol. 33, No. 12, Dec. 1992, Bethesda by P.J. Casey, pp. 1731-1740.
Cohen Louis Hartog
Nieuwenhuizen Willem
LandOfFree
Reagent and kit for determining isoprenylating activity and inhi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Reagent and kit for determining isoprenylating activity and inhi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Reagent and kit for determining isoprenylating activity and inhi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1510896