Reagent and assay methods including a phenazine-containing indic

Details Reagent and assay methods including a phenazine-containing indic Reagent and assay methods including a phenazine-containing indic

1996-02-07

1998-01-13

Leary, Louise

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 25, 435 26, 435 28, 435 15, 435 18, 435 20, 435 791, 436 63, 544347, C12Q 154, C12Q 126, C12Q 128, C07D24146

Patent

active

057078203

ABSTRACT:
A new reagent and methods for measuring the concentration of (or detecting the presence of) an analyte in a sample. The reagent includes a phenazine-containing compound and an enzyme. The phenazine-containing compound must be of sufficient type to form a semiquinoid (the color indicator) by reaction involving the enzyme, analyte, and phenazine-containing compound. Importantly, the phenazine-containing compound must be in sufficient amount to correlate the concentration of semiquinoid to the concentration of analyte in the sample or to detect the presence of the analyte in the sample. The reagent may further include a buffer and a surfactant. The reagent may be incorporated into a film and may be provided in kit form.
The methods for measuring the amount of (or detecting the presence of) an analyte in a sample importantly include spectrophotometric measurement (or detection) of the semiquinoid indicator at wavelengths greater than about 580 nanometers, which reduces interferences due to the presence of hemoglobin, bilirubin, and turbidity. Further, these assay methods importantly involve short incubation periods of less than about one minute for test samples measuring (or detecting) spectrophotometric absorbance (assays performed on solutions) and less than about 1.5 minutes for test samples measuring (or detecting) spectrophotometric reflectance or transmittance (assays performed on films).

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