Reading frame independent epitope tagging

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...

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435 691, 4351723, 4352523, 4353201, 435410, 536 231, 536 243, C12N 1563, C12N 1585, C07H 2104

Patent

active

059486775

ABSTRACT:
Oligonucleotide sequence comprising a repeating nucleotide sequence encoding circularly permuted epitope tag, and vectors comprising the oligonucleotide sequences. Methods for using the sequences to tag proteins. Antibodies specific for the epitopes. Methods for detecting and purifying proteins.

REFERENCES:
patent: 5612180 (1997-03-01), Brown et al.
patent: 5652128 (1997-07-01), Jarvik
Vinay Kumar, Basic Immunology, in "Pathologic Basis of Disease" by Robbins and Cotran, 2d Ed., pp. 262-263 (1979).
Lehninger, Biochemistry, 1st Ed, pp. 652-654, 691-692, 717-718 (1970).

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