Reading, detection or quantification method, hybrids or...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100, C435S288700

Reexamination Certificate

active

07553621

ABSTRACT:
The invention relates to a method of reading, detecting or quantifying at least one biological reaction, on a support, between either a recognition molecule and a labeled target molecule or between a target molecule and a labeled detection molecule. The inventive method comprises treating the support under physicochemical conditions that allow the following: either the separation of the recognition molecule and the labeled target molecule or the separation of the target molecule and the labeled detection molecule. The inventive method further comprises producing images before and after the physicochemical treatment that can be used to determine the specific and non-specific bindings between the different molecules. The invention also relates to hybrids and complexes used in the inventive method and to a biochip containing the same which is used to carry out the inventive method. The invention is particularly suitable for use in the field of diagnosis.

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