Readable probe array for in vivo use

Details Readable probe array for in vivo use Readable probe array for in vivo use

1999-01-19

2001-09-11

Lateef, Marvin M. (Department: 3737)

Surgery

Diagnostic testing

Measuring or detecting nonradioactive constituent of body...

C600S407000, C600S476000, C600S312000, C600S317000, C600S478000, C604S019000, C604S020000, C604S021000, C604S022000, C604S035000, C604S036000, C422S082050, C422S082060, C422S082110, C436S086000, C436S087000, C436S088000, C436S089000, C435S173300, C435S173300

Reexamination Certificate

active

06289229

ABSTRACT:

BACKGROUND OF THE INVENTION
Polydeoxynucleotide and oligonucleotide sequencing with laboratory-based instruments has become inexpensive and reliable due to the variety and availability of complimentary fluorescent labeled target sequences. These fluorescent labeled probes may be specially tailored to hybridize with genomic DNA segments and form base pair matches that can accurately detect the presence of inherited genetic disorders or native-cell mutations. Under excitation light in the visible or UV range, the associated fluorescent marker attached to the probe emits a secondary emission which may be detected by a charge-coupled device (CCD) array, photodiode, or other spectrally sensitive light detector.
However, current techniques require the use of specialized reagents and additional processing to separate the cell wall and other components before analysis. The analyte is removed and introduced into an assay chamber for analysis. The chambers are housed in portable or tabletop analytic instruments that typically contain an excitation source, detection sensors, spatial reading or imaging devices, and archiving capabilities. These systems are expensive and require that tissue samples be processed prior to use. The biggest drawback to these types of systems is their inherent inability to perform fast, localized reading of array probes in a convenient, and repeatable manner in vivo. In vivo monitoring and detection of changes to the human body in response to therapy is needed to expedite trials and to monitor results from therapy, and would allow doctors to treat serious diseases such as cancer safely in a more effective and less costly manner.
SUMMARY OF THE INVENTION
The present invention performs specific detection and analysis of biological analytes in vivo using a simplified, low cost set of components. In one embodiment the small size and simplified operation allows the entire device to be housed in a catheter. In one aspect, the device consists of a housing, a light excitation source, and detector and at least one fluorescent labeled probe material on a substrate that is exposed to the tissue of the body. The excitation source may be directed at the substrate carrying the probe, or may be a conductor of the excitation energy. Other embodiments include the use of a lumen to introduce a lysing agent or energy to the area of interest. The lysing agent or energy may be an ultrasonic transducer capable of rupturing cell membranes through the use of a brief burst of ultrasonic energy. In another aspect, a lysing system is used in which pressurization and evacuation of the sample via the lumen adjacent to the probe array creates a pressure capable of rupturing the cell membrane. Each of the probes may be read by application of electrical current to the excitation source and by detecting the presence or absence of signal via the probe sensor. The probe sensor may be a photodiode that is responsive to light emitted by the fluorescent probe material. Two probes may be mixed and read by two sensors if the spectrum is sufficiently separated. A ratio can then be obtained to facilitate analysis. In another embodiment, a normalizing patch may be adjacent to provide a reference signal, thereby simplifying the calibration of the instrument.


REFERENCES:
patent: 2002559 (1935-05-01), Wappler
patent: 2582937 (1952-01-01), Fossati
patent: 3176114 (1965-03-01), Kneisley
patent: 4233493 (1980-11-01), Nath
patent: 4274706 (1981-06-01), Tangonan
patent: 4289966 (1981-09-01), Roberts
patent: 4340307 (1982-07-01), Diamond et al.
patent: 4472728 (1984-09-01), Grant et al.
patent: 4541272 (1985-09-01), Bause
patent: 4548505 (1985-10-01), Ono
patent: 4560286 (1985-12-01), Wickersheim
patent: 4566057 (1986-01-01), Hinima et al.
patent: 4570638 (1986-02-01), Stoddart et al.
patent: 4578061 (1986-03-01), Lemelson
patent: 4672972 (1987-06-01), Berke
patent: 4718417 (1988-01-01), Kittrell et al.
patent: 4803992 (1989-02-01), Lemelson
patent: 4813790 (1989-03-01), Frankel et al.
patent: 4832034 (1989-05-01), Pizziconi et al.
patent: 4872458 (1989-10-01), Kanshira et al.
patent: 4882623 (1989-11-01), Uchikubo
patent: 4894547 (1990-01-01), Leffell et al.
patent: 4895156 (1990-01-01), Schulze
patent: 4898175 (1990-02-01), Noguchi
patent: 4902896 (1990-02-01), Fertig, Sr. et al.
patent: 4925268 (1990-05-01), Iyer et al.
patent: 4928172 (1990-05-01), Uehara et al.
patent: 4930516 (1990-06-01), Alfano et al.
patent: 4938602 (1990-07-01), May et al.
patent: 4981138 (1991-01-01), Deckelbaum et al.
patent: 5000901 (1991-03-01), Iyer et al.
patent: 5001556 (1991-03-01), Nakamura et al.
patent: 5009655 (1991-04-01), Diagnault, Jr. et al.
patent: 5021888 (1991-06-01), Kondou et al.
patent: 5034010 (1991-07-01), Kittrell et al.
patent: 5036853 (1991-08-01), Jeffcoat et al.
patent: 5042494 (1991-08-01), Alfano
patent: 5045056 (1991-09-01), Behl
patent: 5056503 (1991-10-01), Nagasaki et al.
patent: 5062428 (1991-11-01), Chance
patent: 5106387 (1992-04-01), Kittrell et al.
patent: 5115137 (1992-05-01), Andersson-Engels
patent: 5116759 (1992-05-01), Klainer et al.
patent: 5125404 (1992-06-01), Kittrell et al.
patent: 5127407 (1992-07-01), Tan
patent: 5131398 (1992-07-01), Alfano et al.
patent: 5166755 (1992-11-01), Gat
patent: 5172693 (1992-12-01), Doody
patent: 5174297 (1992-12-01), Daikuzono
patent: 5187572 (1993-02-01), Nakamura et al.
patent: 5187672 (1993-02-01), Chance et al.
patent: 5193542 (1993-03-01), Missanelli et al.
patent: 5197470 (1993-03-01), Helfer et al.
patent: 5201318 (1993-04-01), Rava et al.
patent: 5206174 (1993-04-01), Gehrke et al.
patent: 5213569 (1993-05-01), Davis
patent: 5233621 (1993-08-01), Lawandy
patent: 5242437 (1993-09-01), Everett et al.
patent: 5261410 (1993-11-01), Alfano et al.
patent: 5262645 (1993-11-01), Lambert
patent: 5304178 (1994-04-01), Kittrell et al.
patent: 5305748 (1994-04-01), Wilk
patent: 5309907 (1994-05-01), Fang et al.
patent: 5318024 (1994-06-01), Kittrell et al.
patent: 5348018 (1994-09-01), Alfano et al.
patent: 5350375 (1994-09-01), Deckelbaum et al.
patent: 5351532 (1994-10-01), Hager
patent: 5377676 (1995-01-01), Vari et al.
patent: 5383467 (1995-01-01), Auer et al.
patent: 5386827 (1995-02-01), Chance et al.
patent: 5398844 (1995-03-01), Zaslausky et al.
patent: 5402778 (1995-04-01), Chance
patent: 5402792 (1995-04-01), Kimura
patent: 5402801 (1995-04-01), Taylor
patent: 5405369 (1995-04-01), Selman et al.
patent: 5408998 (1995-04-01), Mersch
patent: 5413108 (1995-05-01), Alfano
patent: 5417207 (1995-05-01), Young et al.
patent: 5417210 (1995-05-01), Funda et al.
patent: 5419323 (1995-05-01), Kittrell et al.
patent: 5421337 (1995-06-01), Richards-Kortum
patent: 5421339 (1995-06-01), Ramanujam et al.
patent: 5445608 (1995-08-01), Chen et al.
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5452723 (1995-09-01), Wu et al.
patent: 5456252 (1995-10-01), Vari et al.
patent: 5461229 (1995-10-01), Sauter et al.
patent: 5467767 (1995-11-01), Alfano et al.
patent: 5512757 (1996-04-01), Cederstrand et al.
patent: 5517313 (1996-05-01), Colvin, Jr.
patent: 5517997 (1996-05-01), Fontenot
patent: 5540691 (1996-07-01), Elstrom et al.
patent: 5542928 (1996-08-01), Evans et al.
patent: 5545897 (1996-08-01), Jack
patent: 5553614 (1996-09-01), Chance
patent: 5555885 (1996-09-01), Chance
patent: 5556421 (1996-09-01), Prutchi et al.
patent: 5562100 (1996-10-01), Kittrell et al.
patent: 5571152 (1996-11-01), Chen et al.
patent: 5579773 (1996-12-01), Vo-Dinh et al.
patent: 5596988 (1997-01-01), Markle et al.
patent: 5626139 (1997-05-01), Szeles et al.
patent: 5632740 (1997-05-01), Koch et al.
patent: 5636612 (1997-06-01), Mitsuhashi et al.
patent: 5647368 (1997-07-01), Zeng et al.
patent: 5679512 (1997-10-01), Laney et al.
patent: 5690894 (1997-11-01), Pinkel et al.
patent: 5730134 (1998-03-01), Dumoulin et al.
patent: 5769791 (1998-06-01), Benaron et al.
patent: 5785658 (1998-07-01), Benaron et al.
patent: 5800478 (1998-09-01), Chen et al.
patent: 5807261 (1998-09-01), Benaron et al.
patent: 5829878 (1998-11-01), Weiss et al.
patent: 5861253 (1999-01-01), Asgari et al.
patent:

Affiliated with

Also associated with

Rating

Readable probe array for in vivo use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Readable probe array for in vivo use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Readable probe array for in vivo use will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2464723