Reactive probes for two-photon fluorescent imaging and sensing

Details Reactive probes for two-photon fluorescent imaging and sensing Reactive probes for two-photon fluorescent imaging and sensing

2007-08-07

2007-08-07

McKane, Joseph K. (Department: 1626)

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

Reexamination Certificate

active

11128497

ABSTRACT:
Fluorescent dyes and probes are key components in multiphoton based fluorescence microscopy imaging of biological samples. In order to address the demand for better performing dyes for two-photon based imaging, a new series of reactive fluorophores tailored for multiphoton imaging has been disclosed. These fluorophores are based upon the fluorene ring system, known to exhibit high fluorescence quantum yields, typically >0.7, and possess high photostability. They have been functionalized with moieties to act, e.g., as efficient amine-reactive fluorescent probes for the covalent attachment onto, e.g., proteins and antibodies. The synthesis and the single-photon spectral characteristics, as well as measured two-photon absorption cross sections of the reactive fluorophores in solution are presented. Spectral characterizations of bovine serum albumin (BSA) conjugated with the new reactive probe is presented.

REFERENCES:
Belfield, et al., “Reactive two-photon fluorescent probes for biological imaging”, Aug. (2003), pp. 91-95, Proceedings of SPIE, vol. 5211.
Katherine Schafer, et al., “Fluorescent dyes for multiphoton bio-imaging applications”, Dec. (2004), Proceedings of SPIE, vol. 5329.
Kevin Belfield, et al., “Reactive two-photon fluorescent probes for biological imaging”, Dec. (2004), pp. 91-95, Proceedings of SPIE, vol. 5329.

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