Reactive derivatives of sulforhodamine 101 with enhanced hydroly

Details Reactive derivatives of sulforhodamine 101 with enhanced hydroly Reactive derivatives of sulforhodamine 101 with enhanced hydroly

1995-06-07

1998-08-25

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

435 6, 435 725, 435 731, 435 732, 436519, 436520, 436529, 436537, 436800, 5303915, 530402, 530404, G01N 33533

Patent

active

057982767

ABSTRACT:
The invention describes reactive dyes having an alkyl spacer attached via a sulfonamide bond to a sulforhodamine 101 fluorophore, and a variety of useful conjugates prepared therefrom. The increased length of the covalent linkage due to the alkyl spacer results in dye-conjugates having a number of surprisingly advantageous properties relative to previous sulforhodamine 101-labeled conjugates, including enhanced solubility and increased fluorescence. The reactive dyes of the present invention are more stable than the known compound sulforhodamine 101 sulfonyl chloride. Novel reactive dyes are described for selective modification of groups other than amines, including thiols and photoreactive derivatives.

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