Reaction cell for protein sequencer and the like

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing gas sample

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422102, 935 86, B01L 1100

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active

053993179

ABSTRACT:
The present invention comprises a reaction cell for a sequencer and the like which includes a sample carrier assembly having a flexible, resilient spacer with a hole therein for retaining the sample on a membrane, a pair of alumina cells which bracket the spacer, one or more pads or spacers to retain the assembly in a tight fitting relation and a cap to secure the assembly together. The reagents are input and withdrawn from opposite edges of the sample membrane. The present invention has a minimal dead volume as a result of the spacer arrangement. It also permits benchtop sample loading due to the separation of the assembly into a carrier and base housing. The fluid lines are self-sealing to the reaction cell.

REFERENCES:
patent: 5106583 (1992-04-01), Raysberg et al.
patent: 5147606 (1992-09-01), Charlton et al.
patent: 5188968 (1993-02-01), Kano et al.
patent: 5200152 (1993-04-01), Brown
Reinke, H., Fischer, St., Reimann, F., and Tschesche, H., Direct Microsequencing of Blotted and Covalently Attached Proteins in a Cross-Flow Reaction Chamber, Methods in Protein Sequence Analysis, pp. 55-66, Birkhauser Verlag, Basil, 1991.
Fischer, St., Reimann, Frank, and Wittmann-Liebold, Brigitte, 3.4 A New Modular Sequencer. 1989.
Sheer, D. G., Yuen, S., Wong, J., Wasson, J., and Yuan, P. M., A Modified Reaction Cartridge for Direct Protein Sequencing on Polymeric Membranes, BioTechniques, vol. 11, No. 4, pp. 526-533. Oct. 1991.
Totty, Nicholas F., Waterfield, Michael D., and Hsuan, J. Justin, Accelerated High-sensitivity Microsequencing of Proteins and Peptides Using a Miniature Reaction Cartridge, Protein Science, I, 1215-24, Cambridge University Press, 1992.

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