Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-11-13
2000-09-19
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 74, 4351885, C12Q 125
Patent
active
061210072
ABSTRACT:
Disclosed and claimed are methods for selecting a recombinant virus, phage or cell expressing a catalytic antibody or catalytic portion thereof, or for selecting catalytic activity by a moiety. The method employs reaction-based selection for catalytic activity. The method can also be used to concentrate (increase the proportion of catalytic to non-catalytic moieties) a sample containing a catalytic moiety or viruses, phages or cells expressing a catalytic moiety. The selection or concentrating can be by employing a mechanism-based inhibitor, catalysis-accelerated movement, surface binding, changes in enthalpic component of binding as a function of temperature, or changes in binding by competition, or combinations thereof. The invention also comprehends a method for producing a recombinant virus or a cell-line expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof; and, this method can include infecting a suitable host with viruses which are screened for the expression. In addition, recombinant viruses and cell-lines so expressing a catalytic moiety such as a catalytic antibody or catalytic portion thereof are also disclosed and claimed.
REFERENCES:
Boder, E. T., et. al. (1997) Nature Biotechnology 15, 553-5.57.
Kieke, M. C., et. al. (1997) Protein Engineering 10(11), 1303-1310.
Georgiou, G., et. al. (1997) Nature Biotechnology 15, 29-34.
Barbas III et al., Assembly of Combinatorial Antibody Liebraires on Phage Surfaces: the Gene III Site, Proc. Natl. Acad. Sci., USA (1991) 88; 7978-82.
Clackson, T., et al., Making antibody fragments using phage display libraries, Nature (1991), vol. 352 pp. 624-628.
Henis et al., "Mobility of Enzymes on Insoluable Substrates" the .beta.-Amylase-Starch Gel System, Biopolymers, vol. 27 pp. 123-138 (1988).
Henis, Y.I., et al. (1988) Chem. Abstracts, 108:127440x.
Huse, W. et al., Generation of a Large Combinatorial Library of the Immunoglobulin Repertoire in Phage Lambda, Science, vol. 246, (Dec. 1989), pp. 1275-1284.
Katchalski-Katzir et al., Enzyme Diffusion and Action on Soluble and Insoluble Substrate Biopolymers, Biopolymers, vol. 24. pp. 257-276 (1985).
Katchalski-Katzir, et al., (1985) Chem. Abstracts, 102:145236r.
McCafferty, et al., Phage antibodies: filamentous phage displaying antibody Variable domains, Nature (1990) 348 552-554.
Richter, P.H. and Eigen, M., Diffusion Controlled Reaction. Rates in Spheroidal-Geometry. Application to Repressor-Operator Association and Membrane Bound Enzymes, Biophysical Chemistry 2 (1974) 255-263.
Richter, P.H., et al., (1974) Chem. Abstracts, 53051s.
Blackburn Gary F.
Darsley Michael J.
Martin Mark T.
Simpson David M.
Smith Rodger G.
IGEN International Inc.
Patterson Jr. Charles L.
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