Reacting an enzyme in a non-aqueous solvent by adding a lyophili

Details Reacting an enzyme in a non-aqueous solvent by adding a lyophili Reacting an enzyme in a non-aqueous solvent by adding a lyophili

1994-03-01

1995-09-12

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

435183, 435195, 435189, 435232, 435233, 435193, C12P 100, C12N 900, C12N 914, C12N 902

Patent

active

054496136

ABSTRACT:
A method for reacting an enzyme in a non-aqueous organic solvent is disclosed. The method comprises preparing a lyophilizate of a salt which activates the enzyme and an enzyme wherein the lyophilizate contains a weight ratio of salt to enzyme of at least 60% salt sufficient to activate the enzyme in an organic solvent. The method then calls for dispersion of the lyophilizate in a non-aqueous organic solvent in the presence of a substrate for the enzyme.

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