Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2006-03-21
2006-03-21
Chen, Stacy B. (Department: 1648)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C435S069100, C424S207100, C536S023100, C536S023720
Reexamination Certificate
active
07015035
ABSTRACT:
This invention provides a retroviral packaging cell comprising a suitable mammalian cell having therein (i) a first recombinant nucleic acid comprising MMLV gag and pol genes and a selectable marker, and (ii) a second recombinant nucleic acid comprising RD114 envelope gene and a selectable marker, wherein the MMLV gag and pol genes and the RD114 envelope gene are stably expressed in the cell. This invention further provides related production methods, virions and kits.
REFERENCES:
patent: 5681746 (1997-10-01), Bodner et al.
patent: 5952225 (1999-09-01), Pensiero et al.
patent: 6372502 (2002-04-01), Bank et al.
Ward et al. Establishment of a Stable RD114 Retroviral Packaging Cell Line that Efficiently Transduces Human Hematopeietic Progenitors, Blood, Nov. 16, 2001, vol. 98, No. 11, Part 2, pp. 408b.
Burns J., et al. (1993) Vesticular stomatitius virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and non-mammalian cells. Proc. Natl. Acad. Sci. USA 90:8033-8037.
Cone, R.D. and Mulligan, R.C. (1984) High-efficiency gene transfer into mammalian cells: generation of helper-free recombinant retrovirus with broad mammalian host range. Proc. Natl. Acad. Sci. USA 81:6349-6353.
Cosset F.L., et al., (1995) High-titer packaging cells producing recombinant retroviruses resistant to human serum. J. Virology 69:7430-7436.
Gatlin J., et al. (2001) Engraftment of NOD/SCID Mice with human CD34+ Cells transduced by concentrated oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD114) Envelope Protein. J. Virology 75:9995-9999.
Gerin et al. (1999) Improved titers of retroviral vectors from the human FLYRD18 packaging cell line in serum-and protein-free medium. Human Gene Ther. 10:1965-1974.
Kaubisch A., et al. (1999) Up-regulation of amphotrophic receptor expression in human peripheral blood CD34+ cells. Am. J. Hematol. 61:243-253.
Kavanaugh M.P., et al. (1994) Cell-surface receptors for gibbon ape leukemia virus and amphotrophic murine retrovirus and inducible sodium-dependent phosphate symporters. Proc. Natl. Acad. Sci. USA 91:7071-7075.
Kelly, P.F., et al. (2000) Highly efficient gene transfer into cord blood nonobese diabetic/severe combined immunodeficiency repopulating cells by oncoretroviral vector particles pseudotyped with the feline endogenous retrovirus (RD114) envelope protein. Blood 96:1206-1214.
Markowitz, et al. (1988) A safe packaging line for gene transfer: separating viral genes on two different plasmids, J. Virology 62:1120-1124.
Markowitz D., et al. (1988) Construction and use of a safe and efficient amphotropic packaging cell line. Virology 167:400-406.
Miller, A.D. and Buttimore, C. (1986) Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Mol. Cell. Biol. 6:2895-2902.
Orlic D., et al. (1996) The level of mRNA encoding the amphotropic retrovirus receptor in mouse and human hermatopoietic stem cells is low and correlates with the efficiency of retrovirus transduction. Proc. Natl. Acad. Sci. USA 93:11097-11102.
Pensiero M. N., et al. (1996) Development of Amphotropic Murine Retrovirus Vectors Resistant to Inactivation by Human Serum. Human Gene Therapy 7:1095-1101.
Porter C. D., et al. (1996) Comparison of Efficiency of Infection of Human Gene Therapy Target Cells via Different Retroviral Receptors. Human Gene Therapy 7:913-919.
Raftopoulos H., et al. (1997) Long-Term Transfer and Expression of the Human β-Globin Gene in a Mouse Transplant Model. Blood 90(9):3414-3422.
Sorge, J., et al. (1984) Amphotropic retrovirus vector system for human cell gene transfer. Mol. Cell. Biol. 4:1730-1737.
Takeuchi, Y., et al. (1994) Type C retrovirus inactivation by human complement is determined by both the viral genome and the producer cell. J. Virology 68:8001-8007.
Ward M., et al. (1994) Transfer and Expression of the Multiple Drug Resistance Gene in Human CD34 Cells. Blood 84(5):1408-1414.
Ward M., et al. (1996) Retroviral Transfer and Expression of the Human Multiple Drug Resistance (MDR) Gene in Peripheral Blood Progenitor Cells. Clinical Cancer Research 2:873-876.
Bank Arthur
Ward Maureen
Chen Stacy B.
Cooper & Dunham LLP
The Trustees of Columbia University in the City of New York
White, Esq. John P.
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