Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Reexamination Certificate
2005-03-29
2005-03-29
Saucier, Sandra E. (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
C435S183000, C435S219000
Reexamination Certificate
active
06872544
ABSTRACT:
A method for assaying the proteinase inhibitor content of tissue of a plant. The proteinase inhibitor and other protein products are extracted from the plant tissue by preparing a mixture of solvent and comminuted plant tissue to form a solid fraction and a liquid fraction which contains the proteinase inhibitor and other protein products. The liquid fraction is heated to a temperature and for a time period sufficient to denature at least some of the other protein products without substantially denaturing the proteinase inhibitor. The denatured, undesired protein products are removed by centrifugation or filtering to prepare a clarified extract solution. The amount of proteinase inhibitor inhibitor in the clan ed extract is measured. If the assay is used in the selection of plant tissues for use in the extraction of proteinase inhibitor, plant tissues with a proteinase inhibitor content less than a pre-selected standard are rejected.
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Fallert Hal G.
Stomp Robert
Davis Law Firm
Herink Kent A.
Kemin Consumer Care, L.C.
Rosenberg Daniel A.
Saucier Sandra E.
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