Ratiometric fluorescent pH sensor for non-invasive monitoring

Details Ratiometric fluorescent pH sensor for non-invasive monitoring Ratiometric fluorescent pH sensor for non-invasive monitoring

2008-06-24

2008-06-24

Warden, Jill (Department: 1797)

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Means for analyzing liquid or solid sample

C422S082050, C422S051000, C422S086000, C436S068000, C436S163000, C436S172000

Reexamination Certificate

active

07390462

ABSTRACT:
The present invention provides ratiometric fluorescent pH sensors for non-invasive, continuous monitoring of pH in such applications as fermentation processes. The ratiometric fluorescent pH sensors comprise a fluorescent dye that exhibits a shift in excitation wavelength with a corresponding shift in pH in the local environment of said fluorescent dye. Ratiometric measurements of the emission intensities at dual excitation maxima correlate to pH. Also provided is a fluorescent dye 6-methacryloyl-8-hydroxy-1,3-pyrene disulfonic acid (MA-HPDS). Further provided are systems and methods to non-invasively and continuously monitor pH.

REFERENCES:
patent: 5273716 (1993-12-01), Northrup et al.
patent: 6485703 (2002-11-01), Cote et al.

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