Rate sensing batch analysis method

Textiles: fiber preparation

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23230R, 23230B, 128 2E, 128 2G, 1951035C, 324 30R, G01h 3114

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039335933

ABSTRACT:
A portable, automated chemical analyzer having a polarographic oxygen electrode submerged in a batch of stirred solution. The signal from the electrode is directly differentiated and amplified to produce a signal proportional to the time rate of change of oxygen concentration. The membrane of the polarographic sensor is stretched very tightly over the cathode surface to provide a high signal-to-noise ratio. Methods for analyzing glucose-glucose oxidase, catalase-H.sub.2 O.sub.2 and various other enzyme systems by the polarographic electrode oxygen sensing apparatus include the steps of converting the sensed signal into time rate of change of oxygen concentration and recording the maximum rate of change of oxygen concentration.

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Leland C. Clark, Jr. et al., Annals of the New York Academy of Sciences, V. 102, Art. 1, pp. 29-44 (1962).
H. V. Malmstadt et al., "Electronics for Scientists", pp. 358, 359, 394 & 395 (1963).
D. Keilin et al., Biochem. J., V. 42, pp. 230-238, (1948).

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