Chemistry: analytical and immunological testing – Involving kinetic measurement of antigen-antibody reaction
Patent
1994-12-02
1996-06-18
Housel, James C.
Chemistry: analytical and immunological testing
Involving kinetic measurement of antigen-antibody reaction
436149, 436172, 436501, 436536, 436537, 436544, 436546, 435 4, 435 6, 435 71, 435 75, 422 52, 422 8201, 422 8205, 422 8208, G01N 33557
Patent
active
055277101
ABSTRACT:
The rate of a biomolecular reaction, such as an enzymatic reaction or an affinity binding reaction, is measured using electrochemiluminescence ("ECL"). The reaction is conducted in an electrochemical cell with a mixture of reagents including a luminophore which will relate the concentration of a reactant, a reaction partner or the reaction product of a reaction partner to the ECL intensity. The reaction partner is a reagent which reacts with the reactant and which participates with the luminophore (or its reaction product participates with the luminophore) to cause the emission of ECL. The ECL intensity is modulated with a series of electrical pulses which are applied to the mixture of reagents at a preselected potential and for preselected intervals of time and duration. The ECL intensity is measured at the same intervals to provide a timed series of values (P). The same experiment is repeated except that the modulation is conducted after the reaction has gone to completion to obtain a timed series of values (C). The same experiment is repeated a third time in the absence of the reaction partner to obtain a times series of values (B). The results are normalized (N) using the following formula: ##EQU1## to obtain a series of values N which can be used to plot the time course (concentration vs. time) of the reaction.
REFERENCES:
patent: 5310687 (1994-05-01), Bard et al.
"Label-free Biosensor Technology Visualizes Biomolecular Interactions in Real Time", 8 No. 2, Products & Innovations Biosensors & Bioelectronics, xi-xiv.
Sjolander S. and Urbaniczky C., "Integrated Fluid Handling System for Biomolecular Interaction Analysis", 63 Analytical Chemistry 2338-2355 (1991).
Friguet et al., "Measurements of the True Affinity Constant in Solution of Antigen-Antibody Complexes by Enzyme-Linked Immunosorbent Assay", 77 Journal of Immunological Methods 305-319 (1985).
Hayes Stephanie A.
Leland Jonathan K.
Nacamulli Laurette
Freed Rachel Heather
Housel James C.
IGEN, Inc.
Robinson William R.
Ryan John W.
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