Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1992-06-24
1994-03-08
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 701, 4352401, 530350, C12N 506, C12P 2102
Patent
active
052926567
ABSTRACT:
The present invention relates to a novel cell line isolated from a rat osteosarcoma wherein the cell line has the following characteristics: a) normal p53 gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; b) normal RB-1 gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; c) a normal c-myc gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; d) a normal c-fos gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; e) a deregulated immediate early gene response; f) a canalicular network MATRIGEL.TM. growth pattern; g) tumorigenic in congenitally athymic mice; h) low levels of alkaline phosphatase activity; i) an ability to produce one or more of the following growth factors: 1) a nonheparin binding growth factor, 2) a first heparin binding growth factor, 3) a second heparin binding growth factor, 4) a third heparin binding growth factor, 5) a fourth heparin binding growth factor, 6) a fifth heparin binding growth factor, 7) a sixth heparin binding growth factor; and j) an ability to be serially propagated greater than sixty population doublings.
The present invention also relates to growth factors having the characteristics of growth factors isolated from the rat osteosarcoma cell line.
The present invention further relates to a process for producing such growth factors comprising growing the cells of the present invention in a culture medium and recovering the growth factors.
REFERENCES:
patent: 5035901 (1991-07-01), Anderson et al.
Majeska et al., 1980, Endocrinology, 107, 1491-1503.
Rodan et al., 1988, Cell and Molecular Biol. of Vertebrate Tissue, Ciba Found. Sym. 136.
Amitani et al., 1975, Jpn. J. Cancer Res., 66, 327-329.
Wozney et al., 1988, Science, 242, 1528-1534.
Miller et al., 1990, Cancer Research, 50, 7950-7954.
Huang et al., 1988, Science, 242, 242, 1563-1566.
Ikeda et al, 1989, Jpn. J. Cancer Research, 80, 6-9.
"Bone-inducing agent (BIA) from cultured human Saos-2 osteosarcoma cells," Anderson, H. C., K. Sugamoto, D. C. Morris, H. H. T. Hsu, & T. Hunt, Bone and Mineral, 16 (1992) pp. 49-62.
"Oncogene, Tumor Suppressor Gene, and Growth Signal Transduction Alterations in a Series of Chemically-Induced Rat Osteosarcomas", Isfort, R. J., D. C. Cody, G. Lovell, & C. J. Doersen, Poster Presentation, Seventh Annual Oncogene Meeting, Frederick, Md., Jun. 24, 1991.
"Growth Factors in Development, Transformation, and Tumorigenesis", Cross, M. and T. M. Dexter, Cell, vol. 64, pp. 271-280, (1991).
"Insulin Promotes Growth of the Cultured Rat Osteosarcoma Cell Line UMR-10601:An Osteoblast-Like Cell", Hickman, J. and A. McElduff, Endocrinology, vol. 124, No. 2, pp. 701-706, (1989).
"Negative regulators of cell growth", Wang, J. L. and Y. M. Hsu, TIBS 11 (1986).
"Growth Factors: Mechanism of Action and Relation to Oncogenes", Heldin, C. H. and Westermark, B., Cell, vol. 37, pp. 9-20, (1984).
"Production of An Insulin-like Growth Factor by Osteosarcoma", Blatt, J., C. White, S. Dienes, H. Friedman and T. P. Foley, Jr., Biochemical and Biophysical Research Communications, vol. 123, No. 1, pp. 373-376 (1984).
Chandar, N., B. Billig, J. McMaster and J. Novak, "Inactivation of p53 Gene in Human and Murine Osteosarcoma Cells," British Journal of Cancer, (Feb. 1992), vol. 65, No. 2, pp. 208-214.
Masuda, H., C. Miller, H. P. Koeffler, H. Battifora and M. J. Cline, "Rearrangement of the p53 Gene in Human Osteogenic Sarcomas", Proc. Natl. Acad. Sci., (Nov. 1987), vol. 84, pp. 7716-7719.
Rodan, G. A. and M. Noda, "Gene Expression in Osteoblastic Cells", Critical Review Eukaryotic Gene, Expression, (1991), vol. 1, Issue 2, pp. 85-91.
Smith, H. S., "In Vitro Properties of Epithellal Cell Lines Established From Human Carcinomas and Nonmalignant Tissue", J. Natl. Cancer Inst., (Feb. 1979), vol. 62, No. 2, pp. 225-230.
Sturm, S. A., P. G. Strauss, S. Adolph, H. Hameister and V. Erfle, "Amplification and Rearrangement of c-myc in Radiation-induced Murine Osteosarcomas", Cancer Research, (Jul. 1990), vol. 50, pp. 4146-4153.
Vukicevic, S., F. P. Luyten, H. K. Kleinman and A. H. Reddi, "Differentiation of Canalicular Cell Processes in Bone Cells by Basement Membrane Matrix Components: Regulation by Discrete Domains of Laminin", Cell, (Oct. 1990), vol. 63, pp. 437-445.
Wrana, J. L., T. Kubota, Q. Zhang, C. M. Overall, J. E. Aubin, W. T. Butler and J. Sodek, "Regulation of Transformation-sensitive Secreted Phosphoprotein (SPPI/osteopontin) Expression by Transforming Growth Factor-.beta.", Biochem. J., (1991), vol. 273, pp. 523-531.
Doersen Claus-Jens W.
Isfort Robert J.
Corstanje Brahm J.
Graff IV Milton B.
Hill Jr. Robert J.
Suter David L.
Teng Sally P.
LandOfFree
Rat osteosarcoma cell line OSR-6 does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Rat osteosarcoma cell line OSR-6, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rat osteosarcoma cell line OSR-6 will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-152624