Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1992-06-24
1994-02-15
Hill, Jr., Robert J.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 701, 4352401, 530350, C12N 0506, C12P 2102
Patent
active
052866440
ABSTRACT:
The present invention relates to a novel cell line isolated from a rat osteosarcoma having the following characteristics: a) a normal p53 gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; b) a normal RB-1 gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; c) a normal c-myc gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; d) a normal c-fos gene as compared to a non-tumorigenic Sprague-Dawley rat cell control; e) a normal immediate early gene response following mitogenic stimulation; f) a canalicular Matrigel growth pattern; g) non-tumorigenic in congenitally athymic mice; h) high levels of alkaline phosphatase activity; i) a lack of autocrine growth factor production; and j) an ability to be serially propagated greater than sixty population doublings.
REFERENCES:
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Doersen Claus-Jens W.
Isfort Robert J.
Corstanje Brahm J.
Graff IV Milton B.
Hill Jr. Robert J.
Suter David L.
Teng Sally P.
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