Rat myeloma cell lines

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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424 85, 4351722, 435240, 435948, 935103, 935105, A61K 3900, C12P 2100, C12N 1500, C12N 500, C12R 191

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active

044725000

ABSTRACT:
A rat myeloma cell line which does not express an immunoglobulin chain, YB2/3.0.Ag.20, is prepared from the cell line Y3-Ag 1.2.3 via a hybrid myeloma cell line. This cell line, and variants thereof prepared by passaging and/or cloning the line, may be fused with immunocyte cells from an animal sensitized to an immunogen to produce hybrid myeloma cell lines which provide a source of monoclonal antibodies to said immunogen.

REFERENCES:
patent: 4350683 (1982-09-01), Galfre et al.
Kearney et al; J. Immunol. 123, 1548, (1979).
Shulman et al; Nature 276, 269, (1978).
Kohler and Howe et al; Eur. J. Immunol. 6, 292, (1976).
Wilde et al; Eur. J. Immunol. 10, 462, (1980).
Galfre et al; Nature 277, 131, (1979).
Kohler et al; Eur. J. Immunol. 6, 511, (1976).

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