Rare earth metal cryptates which are not very sensitive to...

Details Rare earth metal cryptates which are not very sensitive to... Rare earth metal cryptates which are not very sensitive to...

2006-08-08

2006-08-08

Riley, Jezia (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S026600, C540S452000, C540S469000, C540S472000, C534S015000

Reexamination Certificate

active

07087384

ABSTRACT:
The invention relates to a process for reducing the fluorescence quenching caused by the measuring medium in a fluorescence assay, by introducing into said medium of rare earth metal cryptates which are not very sensitive to this fluorescence quenching, said rare earth metal cryptates comprising at least one pyridine radical which is substituted one or more times or unsubstituted.The invention also relates to novel rare earth metal cryptates which are not very sensitive to the fluorescence quenching caused by the measuring medium.

REFERENCES:
patent: 5696240 (1997-12-01), Vallarino et al.
patent: 5925744 (1999-07-01), Haener et al.
patent: WO 99 18114 (1999-04-01), None
G. Mathis: “Rare Earth Cryptates and Homogeneous Fluoroimmunoassays with Human Sera”; Clinical Chemistry, US, American Association for Clinical Chemistry; Winston; vol. 39, No. 9, 1993, pp. 1953-1959, XP002125039; ISSN: 0009-9147.
E. Lopez et al.: “Europium (III) Trisbipyridine Cryptate Label for the Time-Resolved Fluorescence Detection of Polymerase Chain Reaction Products Fixed on a Solid Support”; Clinical Chemistry, US, American Association for Clinical Chemistry; Winston, vol. 39, No. 2, 1993, pp. 196-201, XP002125037; ISSN: 0009-9147; p. 197; Figure 1.
Beatrice Alpha; Elke Anklam; Robert Deschenaux; Jean-Marie Lehn; Marek Pietraskiewicz: “Synthesis and Characterization of the Sodium and Lithium Cryptates of Macrobicyclic Ligands Incorporating Pyridine, Bipyridine, and Biisoguinoline Units”; Helv. Chim. Acta, vol. 71, 1988, pp. 1042-1052; XP002165652.

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