Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Patent
1984-06-08
1985-11-26
Richman, Barry S.
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
436 94, 436169, 436174, 436905, G01N 2177, G01N 3352, G01N 3368
Patent
active
045554900
ABSTRACT:
A method using light ("photodevelopment") to develop a metallic silver image of biopolymers, particularly nucleic acids and proteins separated on polyacrylamide gels, whereby it is possible to visualize protein and nucleic acid patterns within 10 minutes after electrophoretic separation. This "photodevelopment" method requires only two solutions: a solution to "fix" the proteins and a solution containing silver ions, which produces an image when exposed to light. This type of protein stain has achieved a sensitivity of about 0.5 ng of protein. DNA separated on polyacrylamide may also be visualized with this stain.
REFERENCES:
patent: 4405720 (1983-09-01), Merril
Merril et al., Electrophoresis, vol. 3, pp. 17-23, 1982.
Merril et al., Anal. Biochem., vol. 110, pp. 201-207, 1981.
Merril et al., Proc. Natl. Acad. Sci. USA, vol. 76, No. 9, pp. 4335-4339, Sep. 1979.
Switzer et al., Anal. Biochem., vol. 98, pp. 231-237, 1979.
Merril et al., Science, vol. 211, pp. 1437-1438, Mar. 1981.
Langford, Basic Photography, Fourth Edition, pp. 161-163, 1977.
Mannheim, Photography Theory and Practice, Completely Revised and Enlarged Edition, pp. 303-330, 1970.
Hill Jr. Robert J.
Richman Barry S.
The United States of America as represented by the Department of
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