Rapid two-stage polymerase chain reaction method for detection o

Details Rapid two-stage polymerase chain reaction method for detection o Rapid two-stage polymerase chain reaction method for detection o

1997-10-07

2000-08-15

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435 72, 435 732, 435 15, 435 29, 435 912, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2104, G01N 3353

Patent

active

061034681

ABSTRACT:
A method for the rapid, high-sensitivity detection of bacteria in a malt beverage comprising liquid and solid components, wherein the method comprises the steps of:
aseptically sampling said malt beverage;
separating the solids in the sample from the liquid by means of a substrate having antibodies attached thereto;
extracting DNA from the separated solids;
subjecting the extracted DNA to a nested polymerase chain reaction, said reaction comprising:

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