Rapid tuberculosis detection method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S004000, C435S007200, C435S253100, C436S501000, C436S518000, C424S130100, C424S139100, C424S163100, C424S164100, C424S168100, C424S184100, C424S185100, C424S234100, C424S248100

Reexamination Certificate

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07919258

ABSTRACT:
Methods for diagnosingMycobacterium tuberculosisinfection in a human that include providing blood serum, contacting the serum with an Eis antigen fixed on a substrate, thereby forming complexes of Eis antigen with a human antibody that binds to the Eis antigen, contacting the antibody/Eis complexes with a labeled anti-human secondary antibody, and measuring a titer of the human antibody bound to the Eis antigen. In addition, statistically significant positive or negative diagnosis of infection withMycobacterium tuberculosisis provided by comparing patient serum antibody titer with a second titer from a negative control blood sample.

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Dahl, John L. et al., “Subcellular Localization of the Intracellular Survival-Enhancing Eis Protein of Mycobacterium tuberculosis”, Infection and Immunity, Jul. 2001, p. 4295-4302, American Society for Microbiology.

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