Rapid-throughput teleost regeneration assay

Multicellular living organisms and unmodified parts thereof and – Method of using a transgenic nonhuman animal in an in vivo...

Reexamination Certificate

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C800S020000

Reexamination Certificate

active

07655832

ABSTRACT:
The present invention provides a method of simultaneously screening at least two candidate agents for an activity affecting regeneration of an embryonic teleost. According to this method, for each agent, a fin of the embryonic teleost is amputated. Next, the amputated teleost is incubated with the candidate agent for a specified period of time. After this period of time, the amputated teleost is imaged. This image is compared to an image of an amputated teleost that was incubated for the specified period of time in the absence of the candidate agent. When comparing the images, a change in morphology of the amputated fin of the embryonic teleost incubated in the presence of the candidate agent compared to the amputated fin of the embryonic teleost raised in the absence of the candidate agent indicates that the candidate agent affects regeneration.

REFERENCES:
patent: 6656449 (2003-12-01), Serbedzija et al.
patent: 7041276 (2006-05-01), Serbedzija et al.
patent: 2004/0261143 (2004-12-01), Mumm et al.
patent: WO 2005037070 (2005-04-01), None
Langheinrich, U., 2003, BioEssays, 25:904-912.
Quint et al, 2002, PNAS, 99:8713-8718.
Geraudie, 1997, Int Jour of Dev Biol, 41:529-532.
Jeanmarie M. Zodrow et al. 2,3,7,8- Tetrachlorodibenzo-p-dioxin Inhibits Zebrafish Caudal Fin Regeneration, Jul. 18, 2003, Toxicological Sciences 76, pp. 151-161 (2003).
Atsushi Kawakami et al. Early Fin Primordia of Zebrafish Larvae Regenerate by a Similar Growth Control Mechanism with Adult Regeneration, Jun. 28, 2004 Developmental Dynamics 231 pp. 693-699 (2004).
Mathew et al., “Aryl Hydrocarbon Receptor Activation Inhibits Regenerative Growth,”Molecular Pharmacology, 69(1):257-265, 2006.

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