Rapid thermal cycling device

Details Rapid thermal cycling device Rapid thermal cycling device

1994-01-10

1995-10-03

Housel, James C.

Chemistry: molecular biology and microbiology

Apparatus

Including condition or time responsive control means

422 50, 422 99, 422104, 435809, 4352866, 935 85, 935 88, C12M 134

Patent

active

054551757

ABSTRACT:
A thermal cycling device having a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a the DNA polymerase chain reaction. Biological samples are placed in glass microcapillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Once a heating cycle is completed, the controller opens a door to the chamber for venting hot air out and cool ambient air is moved in. Temperature versus time profiles corresponding to optimum denaturation, annealing and elongation temperatures for amplification of DNA are achievable by the present invention.

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