Rapid thermal cycle processing methods and apparatus

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Cell membrane or cell surface is target

Reexamination Certificate

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Reexamination Certificate

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07449314

ABSTRACT:
A system and method for processing a lipid membrane bound structure, the method comprising the steps of providing the structure to be processed in a liquid medium; and heating the liquid medium containing the structure at a rate and through a range sufficient to cause a discrete phase transition in at least one of the membranes, such that the membranes fuse. The method may be used to fuse the structure with another structure, or to reduce the integrity of the structure. The apparatus atomizes a medium containing the structure into small droplets and subjects them to an environment containing steam vapor while moving at high velocity, to rapidly increase the droplet temperature to the steam temperature by release of the latent heat of vaporization.

REFERENCES:
patent: 5962288 (1999-10-01), Aksenov et al.
patent: 7037694 (2006-05-01), Aksenov et al.
patent: 2052967 (1996-01-01), None
Cullis et al., “Physical properties and functional roles of lipids in membranes,” Biochemistry of Lipids, Lipioproteins and Membranes, 1996, Chapter 1, pp. 1-33.
Edidin, “Molecular motions and membrane organization and function,” Membrane Structure, 1981, Chapter 2, pp. 37-81.
Davenport et al., “Studies of lipid fluctuations using polarized fluorescence spectroscopy,” Biological and Synthetic Membranes, 1989, pp. 97-106.

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