Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1997-02-28
1998-12-22
Railey, II, Johnny F.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4353201, 536 231, C12N 509, C12N 1563, C12N 1564, C12N 1566
Patent
active
058518088
ABSTRACT:
The present invention provides compositions, including vectors, and methods for the rapid subcloning of nucleic acid sequences in vivo and in vitro. In particular, the invention provides vectors used to contain a gene of interest that comprise a sequence-specific recombinase target site. These vectors are used to rapidly transfer the gene of interest into any expression vector that contains a sequence-specific recombinase target site located downstream of a promoter element so that the gene of interest may be expressed.
REFERENCES:
patent: 5102797 (1992-04-01), Tucker et al.
Hasan et al., Gene 150:51-56 (1994)!.
Holt et al., Gene 133:95-97 (1993).
Elledge et al., Proc. Natl. Acad. Sci. USA 88:1731-1735 (1991).
Brunelli et al., BioTechniques 16(6):1061-1064 (1994).
Stryer, Biochemistry, 2nd ed., W.H. Freeman and Co., San Francisco, CA (1981), p. 610.
Sambrook, J. et al., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, New York (1989) pp. 16.6-16.8.
Maniatis et al., "Regulation of Inducible and Tissue-Specific Gene Expression," Science 236:1237-1245 (1987).
Voss et al., "The role of enhancers in the regulation of cell-type-specific transcriptional control," Trends Biochem. Sci., 11:287-289 (1986).
Dijkema et al., "Cloning and expression of the chromosomal immune interferon gene of the rate," EMBO J. 4:761-767 (1985).
Uetsuki et al., "Isolation and Characterization of the Human Chromosomal Gene for Polypeptide Chain Elongation Factor-1.alpha.*," J. Biol. Chem., 264:5791-5798 (1989).
Kim et al., "Use of the human elongation factor 1.alpha. promoter as a versatile and efficient expression system," Gene 92:217-223 (1990).
Mizushima and Nagata, "pEF-BOS, a powerful mammalian expression vector," Nuc. Acids. Res., 18:5322 (1990).
Gorman et al., "The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection," Proc. Natl. Acad. Sci. USA 79:6777-6781 (1982).
Boshart et al., "A Very Strong Enhancer Is Located Upstream of an Immediate Early Gene of Human Cytomegalovirus," Cell 41:521-530 (1985).
Metcalf et al. (1996) "Conditionally Replicative and Conjugative Plasmids Carrying lacZ.alpha. for Cloning, Mutagenesis, and Allele Replacement in Bacteria," Plasmid 35:1-13.
Ayres et al. (1993) "Precise Deletions in Large Bacterial Genomes by Vector-mediated Excision (VEX): The trfA Gene of Promiscuous Plasmid RK2 is Essential for Replication in Several Gram-negative Hosts," J. Mol. Biol. 230:174-185.
Pal et al. (1986) "P1 Plasmid Replication Role of Initiator Titration in Copy Number Control," J. Mol. Biol. 192:275-285.
Sugiura et al. (1992) "Minimal Essential Origin of Plasmid pSC101 Replication: Requirement of a Region Downstream of Iterons," J. Bacteriol. 175:5993-6001.
Stenzel et al., "The Integration Host Factor of Escherichia coli Binds to Bent DNA at the Origin of Replication of the Plasmid pSC101," (1987) Cell 49:709.
Grindley and Kelley (1976) "Effects of Different Alleles of the E. coli K12 polA Gene on the Replication of Non-transferring Plasmids," Mol. Gen. Genet. 143:311-318.
Mendiola and de la Cruz (1989) "Specificity of Insertion of IS91, an Insertion Sequence Present in alpha-haemolysin plasmids of Escherichia coli," Mol. Microbiol. 3:979.
Francia and Lobo (1996) "Gene Integration in the Escherichia coli Chromosome Mediated by Tn21 Integrase (Int21)," J. Bact. 178:894-898.
Sternberg et al. (1981) "Site-specific Recombination and Its Role in the Life Cycle of Bacteriophage P1," Cold Spring Harbor Symp. Quant. Biol. 45:297-309.
Hoess et al. (1982) "P1 site-specific recombination: Nucleotide sequence of the recombining sites," Proc. Natl. Acad. Sci. USA 79:3398-3402.
Hoess et al. (1984) "Interaction of the bacteriophage P1 recombinase Cre with the recombining site loxP," Proc. Natl. Acad. Sci. USA 81:1026-1029.
Abremski et al. (1983) "Studies on the Properties of P1 Site-Specific Recombination: Evidence for Topologically Unlinked Products following Recombination," Cell 32:1301-1311.
Abremski et al. (1984) "Bacteriphage P1 Site-specific Recombination," Journal of Biological Chemistry 259:1509-1514.
Hoess and Abremski (1985) "Mechanism of Strand Cleavage and Exchange in the Cre-lox Site-specific Recombination System," J. Mol. Biol. 181:351-362.
Cox (1983) "The FLP protein of the yeast 2-.mu.m plasmid: Expression of a eukaryotic genetic recombination system in Escherichia coli," Proc. Natl. Acad. Sci. USA 80:4223-4227.
Meyer-Lean et al. (1987) "Purification of the FLP site-specific recombinase by affinity chromatography and re-examination of basic properties of the system," Nucleic Acids Res. 15:6469-6488.
Babineau et al. (1985) "The FLP Protein of the 2-micron Plasmid of Yeast," J. Biol. Chem. 260:12313-12319.
Gronostajski and Sadowski (1985) "The FLP Protein of the 2-micron Plasmid of Yeast," J. Biol. Chem. 260:12328-12335.
Weisberg et al., "Site-specific recombination in Phage Lambda," In: Lambda II, Hendrix et al. Eds., Cold Spring Harbor Press, Cold Spring Harbor, NY (1983) pp. 211-250.
Leslie and Sherratt (1995) "Site-specific recombination in the replication terminus region of Escherichi coli: functional replacement of dif," EMBO J. 14:1561.
Lu and Churchward (1994) "Conjugative transposition: Tn916 integrase contains two independent DNA binding domains that recognize different DNA sequences," EMBO J. 13:1541-1548.
Mercier et al. (1990) "Structural and Functional Characterization of tnpI, a Recombinase Locus in Tn21 and Related .beta.-Lactamase Transposons," J. Bacteriol. 172:3745-3757.
Flanagan et al. (1989) "Analysis of Inhibitors of the Site-specific Recombination Reaction Mediated by Tn3 Resolvase," J. Mol. Biol. 206:295.
Stark et al. (1989) "Site-Specific Recombination by Tn3 Resolvase: Topological Changes in the Forward and Reverse Reactions," Cell 58:779-790.
Sato et al. (1990) "The cisA Cistron of Bacillus subtilis Sporulation Gene spoIVC Encodes a Protein Homologous to a Site-Specific Recombinase," J. Bacteriol. 172:1092-1098.
Glasgow et al. (1989) "DNA-binding Properties of the Hin Recombinase," J. Biol. Chem. 264:10072-10082.
Hafter et al. (1988) "Enhancer-independent mutants of the Cin recombinase have a relaxed topological specificity," EMBO J. 7:3991-3996.
Malynn et al. Cell (1988) "The scid Defect Affects the Final Step of the Immunoglobin VDJ Recombinase Mechanism," 54:453-460.
Schild et al. (1990) "Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations," Proc. Natl. Acad. Sci. USA 87:2916-2920.
Bai et al. (1996) "SKP1 Connects Cell Cycle Regulators to the Ubiquitin Proteolysis Machinery through a Novel Motif, the F-Box," Cell 86:263-274.
Hoess et al. (1986) "The role of the loxP spacer region in P1 site-specific recombination," Nucleic Acids Res. 14:2287-2300.
Gay et al. (1985) "Positive Selection Procedure for Entrapment of Insertion Sequence Elements in Gram-Negative Bacteria," J. Bacteriol. 164:918-1237.
Gay et al. (1983) "Cloning Structural Gene sacB, Which Codes for Exoenzyme Levansucrase of Bacillus subtilis: Expression of the Gene in Escherichia coli," J. Bacteriol. 153:1424-1431.
Cigan et al. (1988) "Mutational Analysis of the HIS4 Translational Initiator Region in Saccharomyces cerevisiae," Mol. Cell. Biol. 8:2964-2975.
Yoon et al. (1992) "SSL1, a Suppressor of a HIS4 5'-UTR Stem-Loop Mutation, is Essential for Translation Initiation and Affects UV Resistance in Yeast," Genes and Dev. 6:2463.
Kaniga et al., "A wide-host-range suicide vector for improving reverse genetics in Gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica," Gene 109(1):137-141 (1991).
Wang et al., "pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo," Proc. Natl. Acad. Sci. USA 90(16):7874-7878 (1993).
Palazzolo et al., "Phage lambda cDNA cloning vectors for substractive hybridization, fusion-protein synthesis and Cre-loxP automatic plasmid subcloning," Gene 88:25-36 (1990).
Waterhouse et al., "Combinatorial
Elledge Stephen J.
Liu Qinghua
Baylor College of Medicine
Railey II Johnny F.
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