Rapid subcloning using site-specific recombination

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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4353201, 536 231, C12N 509, C12N 1563, C12N 1564, C12N 1566

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active

058518088

ABSTRACT:
The present invention provides compositions, including vectors, and methods for the rapid subcloning of nucleic acid sequences in vivo and in vitro. In particular, the invention provides vectors used to contain a gene of interest that comprise a sequence-specific recombinase target site. These vectors are used to rapidly transfer the gene of interest into any expression vector that contains a sequence-specific recombinase target site located downstream of a promoter element so that the gene of interest may be expressed.

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