Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1990-06-11
1993-04-27
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 29, 4352403, C12Q 100, C12Q 102, C12N 500
Patent
active
052061515
ABSTRACT:
A rapid method for determining the minimum inhibitory concentration of biocides or biocidal agents for use in various microbiological contaminated aqueous systems is disclosed. This rapid technique provides for an opportunity to determine the minimum amount of anti-microbial agents, or biocides either singularly or combination, to control microbiological growth in aqueous streams contaminated by microorganisms. The test involves the use of reduction oxidation indicator dye systems which react to enhanced microbiological activity producing reducing enzymes such as dehydrogenase enzymes. The technique provides an answer within a time period of from about 30 minutes to about 8-10 hours as opposed to normal testing procedure that can require up to 1-2 weeks. The test procedures uses multiple columns of multiple sample wells on a microtitration plate and techniques for transferring prescribed aliquots of contaminated aqueous systems, nutrients, redox indicator dyes, and incremental and serially diluted concentrations of anti-microbial agents.
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Drake James J.
Miller Robert A.
Nalco Chemical Company
Williams Jane A.
Wityshyn Michael G.
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