Chemistry: analytical and immunological testing – Lipids – triglycerides – cholesterol – or lipoproteins
Patent
1995-06-02
1997-05-13
Alexander, Lyle A.
Chemistry: analytical and immunological testing
Lipids, triglycerides, cholesterol, or lipoproteins
436173, 436177, 436811, B01D 5944
Patent
active
056292104
ABSTRACT:
A rapid screening test was used to distinguish between blood of normal infants and that of individuals having the Smith-Lemli-Opitz Syndrome (SLO). Blood was spotted on filter paper and analyzed with no extractions or separations. The method comprises the steps of vaporizing a cholesterol containing organic sample, ionizing the vaporized sample, detecting fragment ions of said vaporized ionized sample, and determining the intensity ratio of said fragment ions for cholesterol/7-dehydrocholesterol in the sample. The method includes performing the analysis by time-of-flight secondary ion mass spectrometry without derivation or chemical steps.
REFERENCES:
patent: 4224031 (1980-09-01), Mee et al.
patent: 5210412 (1993-05-01), Levis et al.
94270409 Medline "Abnormal Bile Acids in the Smith-Lemliopitiz Syndrome" 94 May 1).
Millington et al., "Tandem Mass Spectrometry: A New Method for Acylcarnitine Profiling with Potential for Neonatal Screening for Inborn Errors of Metabolism", J. Inher. Metab. Dis., vol. 13, pp. 321-324, 1990.
Axelson, "Occurrence of isomeric dehydrocholesterols in human plasma", J. Lipid Res., vol. 32, pp. 1441-1448, 1991.
Chace et al., "Rapid Diagnosis of Phenylketonuria by Quantitative Analysis for Phenylalanine and Tyrosine in Neonatal Blood Spots by Tandem Mass Spectrometry", Clin. Chem., vol. 39, pp. 66-71. 1993.
Tint et al., "Defective Cholesterol Biosynthesis Associated with the Smith-Lemli-Opitz Syndrome", N. Eng. J. Med., vol. 330, pp. 107-113, 1994.
Opitz et al., "Cholesterol Metabolism in the RSH/Smith-Lemli-Opitz Syndrome: Summary of an NICHD Conference", Amer. J. Med. Genet., vol. 50, pp. 326-338, 1994.
Opitz et al., "Smith-Lemli-Opitz (RSH) Syndrome Bibliography: 1964-1993", Amer. J. Med. Genet., vol. 50, pp. 339-343, 1994.
Hercules David M.
Naylor Edwin W.
Zimmerman Paul A.
Alexander Lyle A.
Meyers Diane R.
Silverman Arnold B.
University of Pittsburgh of the Commonwealth System of Higher Ed
LandOfFree
Rapid screening test for smith-lemli-opitz syndrome does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Rapid screening test for smith-lemli-opitz syndrome, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid screening test for smith-lemli-opitz syndrome will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1384703