Rapid screening mutagenesis and teratogenesis assay

Details Rapid screening mutagenesis and teratogenesis assay Rapid screening mutagenesis and teratogenesis assay

1993-04-19

2000-08-08

Priebe, Scott D.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435 4, 435 8, 435 29, 4353201, 435455, C12N 510, C12N 1501, C12N 1585, C12Q 168

Patent

active

061000892

ABSTRACT:
A rapid, sensitive, and quantitative method and assay system using transgenic animal cells and adult or embryonic transgenic animals to rapidly screen compounds for mutagenic or teratogenic activity. Each cell from a cell culture, a differentiated tissue from an animal or an animal embryo contains a target gene and a reporter gene, both including an animal promoter/enhancer, a coding sequence and a transcription termination signal, so that they are capable of expression in animal cells. The product of the target gene is capable of regulating expression of the reporter gene through interaction with a regulatory sequence in the reporter gene. In the preferred embodiment, the target gene lacI (lac repressor) is coupled to the reporter gene lacZ encoding beta-galactosidase, which is easily detectable by cytochemical or histochemical procedures. When the animal cell is exposed to a compound causing a mutation either altering expression of the target gene or altering the operator of the reporter gene, the reporter gene is expressed.

REFERENCES:
patent: 4569916 (1986-02-01), Penman et al.
patent: 4792520 (1988-12-01), Stambrook et al.
patent: 4870009 (1989-09-01), Evans et al.
patent: 4873191 (1989-10-01), Wagner et al.
patent: 4873316 (1989-10-01), Meade et al.
patent: 5510099 (1996-04-01), Short et al.
MacGregor et al., Somat. Cell Mol. Gen. 13(3):253-265 (1987).
Wilmut et al., New Scientist, Jul. 7, 1988, pp. 56-59.
Van Brunt, BioTechnology 6(10):1149,1151,1152,1154 (1988).
Ebert, Karl, "vol. I, Comprehensive Biotechnology First Supplement vol. I Animal Biotechnology" Chapter 3 "Gene Transfer Through Embryo Microinjection" (Tufts Univ. Schools of Med. and Vet Med.) (Published with approval of Pergamon Press).
Rafferty, K.A., "Methods in Experimental Embryology of the Mouse" (The Johns Hopkins Press, Baltimore and London).
Oda, et al., Mutation Research 147, 219-229 (1985).
Quillardet, et al., Proc. Natl. Acad. Sci. USA 79, 5971-5975 (1982).
Flint, Teratology of the Limbs, pp. 325, Merker, et al. editor (Walter de Gruyter & Co. Berlin 1980).
Flint, et al., J. Cell Sci. 61, 247 (1983).
Flint et al. Concepts in Toxicology vol. 3, In vitro Embryotoxicity and Teratogenicity Tests, Homburger, et al. editor (Karger,Basel1985).
Toxic. Appl. Pharmac.76, 383 (1984).
Flint et al J. Appl. Toxicol. 4, 109 (1984).
Keller, Molecular Toxicology 1, 261-276 (1987).
Norton and Coffin, Mol. Cell. Biol. 5, 281-290 (1985).
deWet, et al., Mol. Cell. Biol. 7, 725-737 (1987).
Coulondre and Miller, Mol. Biol. 117, 577 (1977).
Miller, Ann. Rev. Genet. 17, 215 (1983).
Hu and Davidson, Cell 48, 555 (1987).
Brown, et al., Cell 49, 63 (1987).
Allen, et al., Nature 333, 852-855 (1988).
DuBridge, et al., Mol. Cell. Biol. 7, p. 379-387 (1987).
Figge, et al., Cell, vol. 52, p. 713-722 (1988).
Flint, et al., Biol. and App. Pharma.76, p. 383-395 (1984).
Fuerst, et al., Proc. Natl. Acad. Sci. USA, vol. 86, pp. 2549-2553 (1989).
Goring, et al., Science, vol. 235, pp. 456-458 (1987).
Lebkowski, et al., Proc. Natl. Acad. Sci. USA, vol. 82, pp. 8606-8610 (1985).
Lebkowski, et al., Mol. and Cell. Biol., vol. 6, pp. 1838-1842 (1986).
Liu, et al., Proc. Natl. Acad. Sci.USA, vol. 86, pp. 9951-9955 (1989).

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