Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1997-09-17
1998-10-06
Kunz, Gary L.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 913, 536 254, 935 20, C12P 1934, C07H 2102
Patent
active
058177981
ABSTRACT:
Provided herein is a rapid method for isolating total RNA from test samples and further for isolating mRNA from test samples.
REFERENCES:
patent: 4483920 (1984-11-01), Gillespie et al.
patent: 4843155 (1989-06-01), Chomczynski
patent: 4847165 (1989-07-01), Wreschner
patent: 5063162 (1991-11-01), Kiefer
patent: 5234809 (1993-08-01), Boom et al.
"QuickPrep.RTM. Total RNA Extraction Kit", Pharmacia Biotech (1996).
Aviv, H., et al., "Purification of Biologicallly Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose", Proc. Nat. Acad. Sci. USA, 69(6):1408-1412 (1972).
Castellino, F.J., et al., "The Denaturing Effectiveness of Guanidinum, Carbamoylguanidinium, and Guanylguanidinium Salts", Biochemistry, 7(11):4135-4138 (1968).
Chirgwin, J.M., et al., "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease", Biochemistry, 18(24):5294-5299 (1979).
Chomczynski, P., "A Reagent for the Single-Step Simultaneous Isolation of RNA, DNA and Proteins from Cell and Tissue Samples", BioTechniques, 15(3):532-536 (1993).
Chomcyznski, P., et al., "Substitution of Chloroform by Bromochloropropane in the Single-Step Method of RNA Isolation", Analytical Biochemistry, 225:163-164 (1995).
Chomczynski, P., et al., "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction", Analytical Biochemistry, 162:156-159 (1987).
Gillespie, D.H., et al., "Dissolve and capture: a strategy for analysing mRNA in blood", Nature, 367:390-391 (1994).
Hornes, E., et al., "Magnetic DNA Hybridization Properties of Oligonucleotide Probes Attached to Superparamagnetic Beads and Their Use in the Isolation of Poly(A) mRNA From Eukaryotic Cells", GATA, 7(6):145-150 (1990).
Kolbe, T., et al., "Detection of bcrablFusion mRNA is Samples of Whole, Unfractionated Blood", Leukemia Research, 18(6):465-468 (1994).
Porath, J., et al., "Metal chelate affinity chromatography, a new approach to protein fractionation", Nature, 258:598-599 (1975).
Thompson, J., et al., "Molecular Hybridization with RNA Probes in Concentrated Solutions of Guanindine Thiocyanate", Analytical Biochemstry, 163:281-291 (1987).
Abbott Laboratories
Kunz Gary L.
Yasger Paul D.
LandOfFree
Rapid RNA isolation procedure in the presence of a transition me does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Rapid RNA isolation procedure in the presence of a transition me, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid RNA isolation procedure in the presence of a transition me will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-79315