Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is a biological cell or cell fragment
Patent
1980-04-14
1983-08-16
Nucker, Christine M.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is a biological cell or cell fragment
436535, 436542, 436820, 436804, 436828, G01N 3354, G01N 3358, G01N 3360
Patent
active
043992293
ABSTRACT:
A stabilized radioimmunoassay product consisting of an antibody protein-bound to the cell wall of a selected bacterium whereby the antibody is irreversibly bound to the protein and remains specific for the antigen against which it was developed. The radioimmunoassay product is also characterized by the fact that the resultant complete product includes a phase of serum protein treatment to isolate the protein sites or other sites of nonspecific reactivity with the labelled antigen such that the non-specific binding of labelled antigen is significantly reduced. Also within the contemplation of the invention is the method of manufacturing the radioimmunoassay product which includes the initial phase of protein binding of the antibody to the selected bacterium and the subsequent serum treatment to isolate previously unutilized protein sites such that when said sites are subsequently exposed to labelled antigen it will be rejected. Further within the contemplation of the invention is the method of utilizing the aforesaid product to totally eliminate the necessity for the customary incubation stages necessitated by prior art radioimmunoassay techniques.
REFERENCES:
patent: 3995018 (1976-11-01), Stoquist
patent: 4143124 (1979-03-01), Masson et al.
patent: 4166106 (1979-08-01), Sedlacek et al.
patent: 4189464 (1980-02-01), Blumberg et al.
patent: 4223005 (1980-09-01), Teodorescu et al.
patent: 4293538 (1981-10-01), McAleer et al.
Natali et al., J. Immunol. Methods, 25, (1979), 255-264.
The Enzyme Center Inc., "IgGSORB-The New ADSORBING AGENT".
Jonsson et al., Radioimmunoassay and Related Procedures in Medicine, vol. II, IAEA, Vienna, 1974, pp. 287-298.
Kearney et al., J. Immunology, vol. 114, No. 4, Apr. 1975, pp. 1143-1146.
Kessler, J. Immunology, vol. 115, No. 6, Dec. 1975, pp. 1617-1624.
Kessler, J. Immunology, vol. 117, No. 5, Part I, Nov. 1976, pp. 1482-1490.
Bell Michael L.
Chung Roy A.
Kelton Arden A.
Immutron, Inc.
Nucker Christine M.
LandOfFree
Rapid radioimmunoassay product and method of making and using sa does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Rapid radioimmunoassay product and method of making and using sa, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid radioimmunoassay product and method of making and using sa will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-815182