Rapid, radiochemical-ligand binding assay for methotrexate

Details Rapid, radiochemical-ligand binding assay for methotrexate Rapid, radiochemical-ligand binding assay for methotrexate

1975-06-30

1976-09-21

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Attached to antibody or antibody fragment or immunoglobulin;...

23230B, 1951037, G01N 3300, G21H 502, A61K 4300

Patent

active

039819833

ABSTRACT:
A radiochemical ligand binding assay for methotrexate is provided. A binder factor comprising a partially purified dihydrofolic acid reductase preparation is employed. The binder factor is conveniently prepared by homogenizing a factor containing animal organ such as liver, and extracting with isotonic saline and ammonium sulfate. A binder cofactor, NADPH.sub.2, is also employed in the binding reaction. The procedure contemplates both direct and sequential assay techniques, and it is not interfered with by vast excesses of many natural folate derivatives.

REFERENCES:
raso et al., Cancer Research, vol. 35, No. 6, 1975, pp. 1407-1410.
Bohuon et al., Clinical Chemica Acta, vol. 57, No. 3, 1974, pp. 263-267.
Levine et al., Res. Commun. Chem. Pathol. Pharmacol., vol. 9, No. 3, 1974, pp. 543-554.
Freedman et al., Journal of Clinical Pathology, vol. 26, No. 4, 1973, pp. 261-267.

Affiliated with

Also associated with

Rating

Rapid, radiochemical-ligand binding assay for methotrexate does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Rapid, radiochemical-ligand binding assay for methotrexate, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid, radiochemical-ligand binding assay for methotrexate will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1413952