Rapid purification of DNA

Details Rapid purification of DNA Rapid purification of DNA

1990-11-13

1993-02-16

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435259, 435270, 435803, 435820, 536 231, 935 19, 935 20, 935 21, C12P 1934, C12N 108, C12N 106

Patent

active

051870835

ABSTRACT:
The present invention provides a method for rapidly obtaining substantially pure DNA from a biological sample containing cells. The method involves gently lysing the membranes of the cells to yield a lysate containing genomic DNA in a high molecular weight form. The lysate is moved through a porous filter to selectively trap the high molecular weight DNA on the filter. The DNA is released from the filter using an aqueous solution to form a solution containing substantially purified DNA, from which the DNA is recovered.

REFERENCES:
patent: 4900677 (1990-02-01), Hewitt
patent: 4997932 (1991-03-01), Reardon et al.
patent: 5063162 (1991-11-01), Kiefer

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