Rapid polymer sequencer

Details Rapid polymer sequencer Rapid polymer sequencer

2011-05-24

2011-05-24

Dejong, Eric S (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C435S283100, C703S012000

Reexamination Certificate

active

07949472

ABSTRACT:
Method and system for rapid and accurate determination of each of a sequence of unknown polymer components, such as nucleic acid components. A self-assembling monolayer of a selected substance is optionally provided on an interior surface of a pipette tip, and the interior surface is immersed in a selected liquid. A selected electrical field is impressed in a longitudinal or transverse direction at the tip, a polymer sequence is passed through the tip, and a change in an electrical current signal is measured as each polymer component passes through the tip. Each measured change in electrical current signals is compared with a database of reference signals, with each reference signal identified with a polymer component, to identify the unknown polymer component. The tip preferably has a pore inner diameter of no more than about 40 nm and is prepared by heating and pulling a very small section of a glass tubing.

REFERENCES:
patent: 6740518 (2004-05-01), Duong et al.
patent: 7258838 (2007-08-01), Li et al.
patent: 2002/0094526 (2002-07-01), Bayley et al.
Akeson, et al., Microsecond Time-Scale Discrimination Among Polycytidylic Acid, Polyadenylic Aci . . . , Biophysical Journal, Dec. 1999, 3227-3233, 77, Biophysical Society.
Blodgett, Films Built by Depositing Successive Monomolecular Layers on.A Solid Surface, Journal of American Chemical Society, 1935, 1007-1022, 57.
Chechik, et al., Reactions and Reactivity in Self-Assembled Monolayers, Advanced Materials, 2000, 1161-1171, 12-16, Wiley-VCH.
Kasianowicz, et al., Characterization of individual polynucleotide molecules using a membrane channel, Proc. Natl. Acad. Sci., Nov. 1996, 13770-13773, 93.
Lahann, et al., A Reversibly Switching Surface, Science, 2003, 371-374, 299.
Langmuir, The Constitution and fundamental Properties of Solids and Liquids. II. Liquids, J. Amer. Chem. Soc., 1917, 1848-1906, 39.
Li, et al., Ion-beam sculpting at nanometre length scales, Letters to Nature, 2001, 166-169, 412, Macmillan Magazines Ltd.
Marziali, et al., New DNA Sequencing Methods, Annu. Rev. Biomed. Eng., 2001, 195-223, 3, Annual Reviews.
Sagiv, Organized Monolayers by Adsorption. 1. Formation and Structure Oleophobic Mixed Monolayers on S . . . , J. Amer. Chem. Soc., 1980, 92-98, 102, American Chemical Society.
Stein, et al., Ion-Beam Sculpting Time Scales, Physical Review Letters, 2002, 276106-1-276106-4, 89-27, The American Physical Society.

Affiliated with

Also associated with

Rating

Rapid polymer sequencer does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Rapid polymer sequencer, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid polymer sequencer will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2699075