Rapid particle detection assay

Details Rapid particle detection assay Rapid particle detection assay

2011-04-19

2011-04-19

Yu, Melanie J (Department: 1641)

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Structured visual or optical indicator, per se

C422S050000, C422S400000, C422S401000, C436S518000, C436S526000, C436S164000

Reexamination Certificate

active

07927561

ABSTRACT:
The present invention proves instruments and methods for detecting and/or quantitating an analyte in a fluid sample. The fluid sample is placed in a sample chamber having a small, shallow detection region. The analyte is magnetically labeled using magnetic particles coated with a binding reagent, and is detectably labeled using a fluorescent dye or other detection reagent. The magnetically labeled analyte is concentrated into the detection region using a focusing magnet positioned underneath the sample chamber detection region. Concentrated analyte is measured using excitation optics positioned on top of the sample chamber detection region, adapted to illuminate only the detection region, and detection optics positioned on top of the detection region, adapted to detect only light emitted from the detection region. In a preferred embodiment, the invention provides a simple, rapid assay for measuring the concentration of CD4+T cells in a whole blood sample.

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