Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-10-11
1999-09-21
Scheiner, Laurie A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 721, 435 737, 435 691, 435 693, 435 9121, 4351721, 4352523, 435270, 4353201, 435325, 436518, 436548, 5303882, C12Q 168
Patent
active
059552646
ABSTRACT:
A rapid mutational analysis method for mapping protein epitopes is disclosed. This method has been used to identify the binding sites for 16 anti-CD2 and anti-CD4 monoclonal antibodies. The powerful, rapid, and simple method of the present invention allows isolation of a very large number of mutants, and is applicable to any intracellular or surface protein for which a cDNA and monoclonal antibodies are available. The present method is especially useful in ligand binding site studies for the design of new ligands and drugs.
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Peterson Andrew
Seed Brian
Scheiner Laurie A.
The General Hospital Corporation
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